We narrowed to 14,919 results for: pET

1. pUG-124

   Plasmid

   #104635

   Purpose

   Level 2 construct

   Depositor

   Marco Trujillo

   Article

   Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

   Insert

   HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4
   

   Use

   Tags

   HIS-Ubq, HA-E2, GST-E3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBD-0016

   Plasmid

   #12345

   Depositor

   Axel Brunger

   Article

   DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

   Insert

   VCP R313K (Vcp Mouse)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   R313K, I206V*(see below)

   Promoter

   Available since

   Aug. 24, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pCri-13a

   Plasmid

   #61321

   Purpose

   Protein expression.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

   Insert

   Green fluorescent protein
   

   Use

   Tags

   His-tag and MISTIC-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDJSVT89

   Plasmid

   #109303

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

   Type

   Empty backbone

   Use

   Tags

   TamA 1-27, 6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. K560_AviN_coBirA

   Plasmid

   #129766

   Purpose

   Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Use

   Tags

   Avi-tag GLNDIFEAQKIEWH and His6

   Expression

   Bacterial

   Mutation

   Truncation at 559

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKEW215_AcrIIA13

   Plasmid

   #138281

   Purpose

   Expression of AcrIIA13 protein

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA13
   

   Use

   CRISPR

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2CT-Cdi

   Plasmid

   #83044

   Purpose

   Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

   Depositor

   Jennifer Doudna

   Article

   Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

   Insert

   CdiCas9
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

8. 6xHis-MBP-huLb5Cas12a

   Plasmid

   #174684

   Purpose

   Expresses 6xHis-MBP tagged Lb5Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huLb5Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KaiB-FLAG

   Plasmid

   #41885

   Depositor

   Michael Rust

   Article

   Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

   Insert

   Kai B
   

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   t7 promoter

   Available since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

10. 6xHis-MBP-huHkCas12a

    Plasmid

    #174683

    Purpose

    Expresses 6xHis-MBP tagged HkCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huHkCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCri-8a

    Plasmid

    #61317

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCri-4b

    Plasmid

    #61328

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and TRX-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCri-11b

    Plasmid

    #61333

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

14. K406

    Plasmid

    #129764

    Purpose

    Expresses Drosophila Kinesin-1 truncated after neck-coil at 406

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Truncation at 406

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Human Mevalonate Kinase (MK)

    Plasmid

    #52429

    Purpose

    bacterial expression of human Mevalonate Kinase (MK)

    Depositor

    Henry Miziorko

    Article

    Potter et al J Biol Chem. 1997 Oct 10;272(41):25449-5

    Insert

    Human Mevalonate Kinase (MVK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    see comments

    Promoter

    T7

    Available since

    April 10, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pUG-301

    Plasmid

    #104730

    Purpose

    Level 2 construct

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    HIS-Ubq_UBA1_HA-UBC10_EL3
    

    Use

    Tags

    HIS-Ubq, HA-E2

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCri-4a

    Plasmid

    #61314

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and TRX-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

18. 6xHis-MBP-huPcCas12a

    Plasmid

    #174681

    Purpose

    Expresses 6xHis-MBP tagged PcCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPcCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 6xHis-MBP-huCMtCas12a

    Plasmid

    #174680

    Purpose

    Expresses 6xHis-MBP tagged CMtCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huCMtCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 6xHis-MBP-huPdCas12a

    Plasmid

    #174682

    Purpose

    Expresses 6xHis-MBP tagged PdCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPdCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge