We narrowed to 5,637 results for: chia

1. ABE8r-R153del

   Plasmid

   #208296

   Purpose

   Expresses ABE8r in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA8r-R153del-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJB042 (FtsZ-mEos2)

   Plasmid

   #49764

   Purpose

   Inducible expression of FtsZ-mEos2 in bacteria

   Depositor

   Jie Xiao

   Article

   Buss et al Mol Microbiol. 2013 Sep;89(6):1099-120.

   Insert

   FtsZ-mEos2
   

   Use

   Tags

   mEos2

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPE1

   Plasmid

   #209981

   Purpose

   Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   E. coli shuttle part (pMB1_ampR_B0017)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLPE2

   Plasmid

   #209982

   Purpose

   Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   E. coli shuttle part (ampR_B0017)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPE3

   Plasmid

   #209985

   Purpose

   Contains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   E. coli shuttle part (pMB1_cat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSCL.139

   Plasmid

   #184994

   Purpose

   Express Cas9-P2A-Eco1RT in human cells

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   SpCas9-P2A-Eco1RT
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   Human codon optimized RT

   Promoter

   TetOn-3g

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-mCherry-2xEcMTS

   Plasmid

   #133624

   Purpose

   Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

   Insert

   mCherry-2xEcMTS
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. P628_SpCas9-RecA

   Plasmid

   #87263

   Purpose

   Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   no

   Promoter

   CBh

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET FLII12Pglu-700uDelta6

   Plasmid

   #13568

   Depositor

   Wolf Frommer

   Article

   Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

   Insert

   FLIPglu F16A Y12S-ECFP-D13R, 6aa linker
   

   Use

   Tags

   Citrine

   Expression

   Bacterial

   Mutation

   F16A, ECFP insert in mglB

   Promoter

   Available since

   Feb. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. eGFP-CBP

    Plasmid

    #169211

    Purpose

    Expression of eGFP-CBP protein for chitin visualization

    Depositor

    James S Fraser

    Article

    Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

    Insert

    Chitinase A1 Chitin Binding Domain
    

    Use

    Tags

    6xHis, Mxe Gyr Intein, and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBEL1203

    Plasmid

    #196024

    Purpose

    Expression/purification of 6xHis-TEV-MlaC

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS2365-Tier1-PhCMV-TetR

    Plasmid

    #169566

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven tetracycline repressor protein class B from transposon Tn10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF-ENTR-LacZ (710-1)

    Plasmid

    #17430

    Purpose

    Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    LacZ
    

    Use

    Entry vector

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEL1092

    Plasmid

    #195635

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSF-1b-S14

    Plasmid

    #128596

    Purpose

    Expresses tagless 30S ribosomal subunit protein S14 in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S14 (rpsN E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHL 246

    Plasmid

    #53014

    Purpose

    AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    fimS (off orientation)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pRT801

    Plasmid

    #68112

    Purpose

    Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.

    Depositor

    Maggie Smith

    Article

    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

    Inserts

    aac3(IV)
    oriT
    attP
    int
    

    Use

    Streptomyces integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET FLIPE intramol-1u

    Plasmid

    #13549

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIP-E intramol WT (gltI E. coli)
    

    Use

    Tags

    EYFP

    Expression

    Bacterial

    Mutation

    ECFP insert in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-Streptavidin-K121R

    Plasmid

    #89880

    Purpose

    Streptavidin that has unimpaired biotin binding after dye labeling

    Depositor

    Mark Howarth

    Article

    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

    Insert

    Streptavidin-K121R-Glutamate_Tag
    

    Use

    Tags

    6 glutamate tag (C terminal on insert)

    Expression

    Bacterial

    Mutation

    K121R

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 FLIPE-1m

    Plasmid

    #13544

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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