We narrowed to 6,250 results for: nls

1. A3G-BE5.13

   Plasmid

   #163637

   Purpose

   C to T DNA base editing

   Depositor

   Xue Gao

   Article

   Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

   Insert

   A3G-BE5.13
   

   Tags

   BPSV40 NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. A3G-BE4.4

   Plasmid

   #163636

   Purpose

   C to T DNA base editing

   Depositor

   Xue Gao

   Article

   Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

   Insert

   A3G-BE4.4
   

   Tags

   BPSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #135012

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Tags

   3x Flag, 3xFLAG (N terminal on insert), NLS, and …

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-hA3A-BE3-Y130F

   Plasmid

   #113428

   Purpose

   Expresses hA3A-BE3-Y130F in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-BE3-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y130F

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

   Plasmid

   #140198

   Purpose

   Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Insert

   dLbCas12a(D156R)-VPR
   

   Use

   CRISPR and Synthetic Biology; Expression in asper…

   Tags

   VPR (VP64-p65-Rta), NLS

   Mutation

   D832A DNase deactivated, D156R for improved activ…

   Promoter

   PgpdA

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. eSpCas9-plus

   Plasmid

   #126767

   Purpose

   Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, R1060A; amino acids 1005-1013 replaced wit…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6_sgRNA_CAG_hSt1Cas9_LMD9

   Plasmid

   #110626

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and hU6

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Fuw-AcrIIA4-P2A-GFP

   Plasmid

   #108247

   Purpose

   Lentiviral vector to express AcrIIA4-P2A-GFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   AcrIIA4-NLS-HA (NEWENTRY Synthetic)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   Ubc

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

   Plasmid

   #113677

   Purpose

   A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   de-catalyzed SaCas9
   

   Use

   AAV and CRISPR

   Tags

   KRAB and NLS

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA

    Plasmid

    #113685

    Purpose

    An EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    inverted de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, Cre/Lox, and Synthetic Biology

    Tags

    NLS and VP64

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLex_Cas9

    Plasmid

    #117987

    Purpose

    pLex_Cas9 is a single insert lentiviral vector expressing Cas9 driven by CMV promoter.

    Depositor

    Paul Khavari

    Article

    Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

    Insert

    Cas9-P2A-NLS-BASTR
    

    Use

    Lentiviral

    Tags

    P2A linker, nuclear localization signal and blast…

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

    Plasmid

    #113681

    Purpose

    A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV and CRISPR

    Tags

    KRAB and NLS

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBUN501

    Plasmid

    #50582

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    zCas9D10A
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Mutation

    Cas9D10A nickase, was derived from the zCas9 and …

    Promoter

    AtU6-26p and Ubi1p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. INS-GFP-CD19-EPT

    Plasmid

    #210469

    Purpose

    Donor plasmid for knock-in GFP-CD19 into the human INS locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. LightR-iCre-miRFP670

    Plasmid

    #162158

    Purpose

    Expresses LightR-iCre-miRFP670 in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    LightR-iCre-miRFP670
    

    Tags

    NLS and miRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEMS1424

    Plasmid

    #29220

    Purpose

    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple155-EGFP-NLS
    

    Use

    High copy number homologous recombination plasmid…

    Expression

    Mammalian

    Promoter

    MiniPromoter Ple155 from the human PCP2 gene

    Available Since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-BE4RA-P2A-Puro

    Plasmid

    #112672

    Purpose

    Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJEP303-pAAV-CMV-dSaCas9-VP64-pA

    Plasmid

    #113678

    Purpose

    A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    NLS and VP64

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SaLgCP

    Plasmid

    #164562

    Purpose

    Puro

    Depositor

    Junwei Shi

    Article

    Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Flag-NLS-SaCas9-P2A-PuroR

    Expression

    Mammalian

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-Flag-LmoCas6

    Plasmid

    #126485

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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