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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 4641 - 4660 of 6562 results

  2. pHS2056

    Plasmid
    #239837
    Purpose
    OMEGAOff + omegaRNA scaffold AAV expression
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    DNMT3A-DNMT3L-NovaIscB-KRAB::NovaIscB omegaRNA scaffold
    Use
    AAV
    Tags
    HA and SV40 NLS
    Mutation
    Insertion of REC domain from NbaCas9-1 + E137K/E4…
    Promoter
    EFS; human U6
    Available since
    July 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239837-plasmid-map-sequence-id-479103

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  4. YE2-BE4max

    Plasmid
    #138156
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YE2)-nSpCas9-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R132E; within Cas9 D10A
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    138156-plasmid-map-sequence-id-272343

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  5. pEGFP-N1_MeCP2(deltaNIC)

    Plasmid
    #110191
    Purpose
    Expression of mouse MeCP2 (deltaNIC) in mammalian cells
    Depositor
    Adrian Bird
    Publication
    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    delta N and C termini. Intervening region replace…
    Promoter
    CMV
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110191-plasmid-map-sequence-id-210799

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  7. BA31_pU.CAG.SaCas9-D10A.rBGpA

    Plasmid
    #199251
    Purpose
    Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaCas9.D10A nickase
    Use
    CRISPR
    Tags
    3XHA epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199251-plasmid-map-sequence-id-391385

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  8. 6xHis-MBP-huBsCas12a

    Plasmid
    #174671
    Purpose
    Expresses 6xHis-MBP tagged BsCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huBsCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    174671-plasmid-map-sequence-id-364005

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  9. pRS416-Gal4-dCas9-VP64

    Plasmid
    #71128
    Purpose
    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.
    Depositor
    Ronald Davis
    Publication
    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.
    Insert
    Gal4-dCas9-VP64
    Tags
    NLS n-terminal, N terminal Gal4 Activator domain,…
    Expression
    Yeast
    Promoter
    Tef1
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    71128-plasmid-map-sequence-id-127063

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  11. pGfa2(ABDx3)-nLac

    Plasmid
    #53133
    Purpose
    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cells
    Depositor
    Michael Brenner
    Publication
    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    -2163 to +47, with 3 copies of 3 copies of the gf…
    Promoter
    human Gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53133-plasmid-map-sequence-id-189228

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  12. pTE3646

    Plasmid
    #137714
    Purpose
    Expresses LbCas12a RVRR variant in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCas12a-RVRR
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    G532R, K538V, Y542R, K595R
    Promoter
    CMV
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137714-plasmid-map-sequence-id-274022

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  13. pEMS1228

    Plasmid
    #29114
    Purpose
    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple246 (VIM Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Nov. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    29114-plasmid-map-sequence-id-13529

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  15. pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

    Plasmid
    #85453
    Purpose
    Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.
    Depositor
    Aviv Regev
    Publication
    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do
    Insert
    U6-sgRNA-Backbone-UP-TdTomato
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    deletion of AA123-365 in tdTomato (please see dep…
    Available since
    April 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    85453-plasmid-map-sequence-id-173968

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  17. PX400 Campylobacter lari CF89-12 Cas9

    Plasmid
    #68336
    Purpose
    Campylobacter lari CF89-12 Cas9
    Depositor
    Feng Zhang
    Insert
    ClCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68336-plasmid-map-sequence-id-119558

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  19. pAT15547_PEmax-SuperFi-Cas9

    Plasmid
    #184377
    Purpose
    Mammalian expression of SuperFi-Cas9 PEmax prime editor
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-SuperFi-Cas9
    Use
    CRISPR
    Tags
    SV40 NLS and c-myc NLS
    Expression
    Mammalian
    Mutation
    H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…
    Promoter
    CMV
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184377-plasmid-map-sequence-id-362139

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Showing: 4641 - 4660 of 6562 results