We narrowed to 5,339 results for: pcdna3.1

1. pcDNA3-N-mSOG-bFos-IRES-mCherry

   Plasmid

   #136599

   Purpose

   Encodes bFos domain tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

   Depositor

   John Ngo

   Article

   Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

   Insert

   N-mSOG-bFos
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-Hu_Plk5(WT)

   Plasmid

   #136319

   Purpose

   Mammalian expression of human Plk5-WT with N-terminal EGFP

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

   Insert

   PLK5 (PLK5 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTE4999

   Plasmid

   #107555

   Purpose

   Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(dead1)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   D917A

   Promoter

   CMV

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Opto-GPR27

   Plasmid

   #106013

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR27; Opto-GPR27; B3)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR27 (GPR27 Bovine, Human)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Opto-GPR161

   Plasmid

   #106055

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR161; Opto-GPR161; E9)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR161 (GPR161 Bovine, Human)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. BE3-NLS-NLS-P2A-BLAST

   Plasmid

   #135348

   Purpose

   Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   BE3-2XNLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. S1 1029 CP ABEMax strategy 1

   Plasmid

   #135357

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   ABEMax-nSpyCas9 aa 1029
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-MED11

   Plasmid

   #15418

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED11 (Med11 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pFLAG-EEF1D (pcDNA 3.1+) LG3-1

   Plasmid

   #37364

   Depositor

   David Litchfield

   Article

   Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

   Insert

   EEF1D (EEF1D Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2012

   Availability

   Academic Institutions and Nonprofits only

10. Opto-GPR37

    Plasmid

    #106019

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR37; Opto-GPR37; B9)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR37 (GPR37 Bovine, Human)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. hHSF1 S303A

    Plasmid

    #118363

    Purpose

    This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S303A

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRD398

    Plasmid

    #163105

    Purpose

    Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mCherry_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Expression

    Mammalian

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA HA-Gnat1 WT-3UTR

    Plasmid

    #184026

    Purpose

    Expresses mouse Gnat1 protein with N-terminal HA tag from cDNA that contains the full length 3'-UTR

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Gnat1 (Gnat1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-MED30

    Plasmid

    #15426

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED30 (Med30 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

15. MAP4K3-GFP K45E

    Plasmid

    #21734

    Depositor

    L. Miguel Martins

    Article

    Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

    Insert

    MAP4K3 (MAP4K3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Mutation of the ATP-binding site (K45E). This ver…

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

16. FLAG-MED20

    Plasmid

    #15421

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED20 (Med20 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pTOPO-MCL1S159A

    Plasmid

    #21606

    Depositor

    Ulrich Maurer

    Article

    Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

    Insert

    MCL-1 S159A (MCL1 Human)
    

    Expression

    Mammalian

    Mutation

    Serine 159 mutated to Alanine, abolishing phosph…

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

18. PA-RL-BAD

    Plasmid

    #78859

    Purpose

    DULIP positive control (bait) for assay establishment

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    BCL2 associated agonist of cell death (BAD Human)
    

    Use

    Expression vector

    Tags

    Protein A, Renilla luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C2_3X Flag

    Plasmid

    #194161

    Purpose

    Expresses eGFP fluorescent protein with a 3X flag tag

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    GFP with 3X Flag
    

    Tags

    3x Flag

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-MED9

    Plasmid

    #15407

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    MED9 (Med9 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only