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pVRa16_3622

Plasmid

#49670

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF16_3622

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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PTEN-mut (negative control)

Plasmid

#86646

Purpose

expression of control shRNA

Depositor

Jaime Modiano

Publication

Lin et al Mol Cancer. 2007 Feb 14;6:16.

Insert

PTEN (control)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

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BA31_pU.CAG.SaCas9-D10A.rBGpA

Plasmid

#199251

Purpose

Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

Depositor

Manuel Goncalves

Publication

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

SaCas9.D10A nickase

Use

CRISPR

Tags

3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMF-FU_TetO_GW_PPARG2

Plasmid

#193289

Purpose

Lentiviral construct that expresses a dox-inducible human PPARG2

Depositor

Rudolf Jaenisch

Publication

Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

Insert

PPARG2 (PPARG2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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OSC_Reporter_UAS_traffic jam_BoxB_d2eGFP_t2a_Blast

Plasmid

#128010

Purpose

traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites

Depositor

Julius Brennecke

Publication

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites

Use

Drosophila osc reporter construct

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Dendra2-Gamma-Tubulin-17

Plasmid

#57721

Purpose

Localization: Centrosomes, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-LRRK2

Plasmid

#23520

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LRRK2 (LRRK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-RP6-213H19.1

Plasmid

#23561

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RP6-213H19.1 (STK26 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PDGFRL

Plasmid

#23591

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRL (PDGFRL Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-FGFRL1

Plasmid

#23600

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGFRL1 (FGFRL1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-CKMT1A

Plasmid

#23706

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CKMT1A (CKMT1A Human)

Use

Gateway Cloning

Mutation

1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pEasyCas9

Plasmid

#184909

Purpose

Expresses the Streptococcus pyogenes Cas9 protein

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

Cas9

Use

CRISPR

Expression

Bacterial and Yeast

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-NME6

Plasmid

#23562

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME6 (NME6 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pIOM15

Plasmid

#20869

Depositor

Carmen Beuzón

Publication

Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

Insert

aphA::aadA

Expression

Bacterial

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only
pET-22b(+)-HpENR

Plasmid

#12651

Depositor

SeWon Suh

Publication

Lee et al Acta Crystallogr D Biol Crystallogr. 200

Insert

noyl-acyl carrier protein reductase (fabI Helicobacter pylori)

Expression

Bacterial

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEMTB-mScarlet3-H (aka mYongHong)

Plasmid

#239407

Purpose

Expresses EMTB-mScarlet3-H in mammalian cells

Depositor

Zhifei Fu

Publication

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

EMTB (MAP7 Human)

Tags

mScarlet3-H (aka mYongHong)

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ZFP36L2

Plasmid

#156149

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ZFP36L2 (ZFP36L2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC19-YAP-mEGFP

Plasmid

#166472

Purpose

HDR template for tagging of endogenous mouse YAP N-terminus with mEGFP

Depositor

Shuguo Sun

Publication

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP-mEGFP HDR template(mouse Yap) (Yap1 Mouse)

Expression

Mammalian

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_WT

Plasmid

#162579

Purpose

Expresses norovirus GI.1 VP1 protein in Sf9 insect cells

Depositor

Peter Kwong

Publication

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Promoter

polyhedrin

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC840 - pAAV 5xATF6 secNLuc

Plasmid

#82497

Purpose

Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

Depositor

Brandon Harvey

Publication

Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

Insert

secNLuc

Use

AAV

Expression

Mammalian

Promoter

5x(ATF6 binding site) c-Fos minimal promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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