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pHAGE-ALK-F1174L

Plasmid

#116108

Purpose

Lentiviral expression of ALK F1174L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ALK (ALK Human)

Use

Lentiviral

Mutation

F1174L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd5

Plasmid

#102867

Purpose

Expresses mouse Fzd5 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 5 (Fzd5 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

A to G substitution at nucleotide 699 of Fzd5 ORF…

Promoter

EF1A

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-A289V

Plasmid

#116230

Purpose

Lentiviral expression of EGFR A289V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

A289V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RAC1

Plasmid

#116783

Purpose

Lentiviral expression of RAC1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RAC1 (RAC1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAP3K4

Plasmid

#116759

Purpose

Lentiviral expression of MAP3K4

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP3K4 (MAP3K4 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLT1

Plasmid

#116744

Purpose

Lentiviral expression of FLT1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT1 (FLT1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q48

Plasmid

#111747

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q48 (polyQ repeat)

Promoter

CMV and P10

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB3-D297Y

Plasmid

#116360

Purpose

Lentiviral expression of ERBB3 D297Y

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

D297Y

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSLED.NPL

Plasmid

#193014

Purpose

Neuron-specific reporter (CBh promoter, Pls3 exon, bichromatic reporter)

Depositor

Jonathan Ling

Article

Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

Insert

Bichromatic reporter (EGFP and dsRed-Express2)

Use

AAV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Homer1L scFv [L113/13]

Plasmid

#190498

Purpose

Mammalian Expression of Homer1L scFV. Derived from hybridoma L113/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Homer1L (Mus musculus) recombinant scFV (Homer1 Mouse)

Tags

Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-A289T

Plasmid

#116229

Purpose

Lentiviral expression of EGFR A289T

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

A289T

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-LAR/PTPRF [N165/38R]

Plasmid

#177504

Purpose

Mammalian Expression Plasmid of anti-LAR/PTPRF (Human). Derived from hybridoma N165/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-LAR/PTPRF (Homo sapiens) recombinant mouse monoclonal antibody (PTPRF Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-AKT1

Plasmid

#116710

Purpose

Lentiviral expression of AKT1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-EGR3-P2A-mCherry

Plasmid

#203861

Purpose

Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

EGR3 (EGR3 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, Alpha1 scFv [N95/35]

Plasmid

#199430

Purpose

Mammalian expression of GABA(A)R, Alpha1 scFv. Derived from hybridoma N95/35.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingGABA(A)R, Alpha1 (Human) (GABRA1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Kv4.2 K+ channel (external) scFv [K57/41]

Plasmid

#206771

Purpose

Mammalian Expression of Kv4.2 K+ channel (external) scFV. Derived from hybridoma K57/41 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv4.2 K+ channel (external) (Homo sapiens) recombinant scFV (KCND2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Neurexin-1-Beta scFv [N170A/26]

Plasmid

#199425

Purpose

Mammalian expression of Neurexin-1-Beta scFv. Derived from hybridoma N170A/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Neurexin-1-Beta (Human) (NRXN1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, Gamma2L/S scFv [N452/73]

Plasmid

#190555

Purpose

Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-M766_A767insAI

Plasmid

#116279

Purpose

Lentiviral expression of EGFR M766_A767insAI

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

M766_A767insAI

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-IRSp53/BAIAP2 [L117/1R]

Plasmid

#177462

Purpose

Mammalian Expression Plasmid of anti-IRSp53/BAIAP2 (Human). Derived from hybridoma L117/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-IRSp53/BAIAP2 (Homo sapiens) recombinant mouse monoclonal antibody (BAIAP2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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CIRTS-FTO-control

Plasmid

#216859

Purpose

CIRTS RNA targeting system for targeted removal of m6A on transcript at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.

Depositor

Timothy Bredy

Article

Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

Insert

CIRTS-FTO-Calm3-U6-control gRNA

Use

Lentiviral

Tags

mCherry2

Expression

Mammalian

Promoter

Syn1, U6

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-FLTID-GSQ-PMLivaWT-P2A-BLAST

Plasmid

#208040

Purpose

Enables constitutive expression of N-terminal TurboID-fused PML (isoform IVa; WT); selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

PML (isoform IVa) (PML Human)

Use

Lentiviral

Tags

FLAG, TurboID

Promoter

EFS

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PSD-95 scFv [K28/74]

Plasmid

#190490

Purpose

Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/74.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR 223 VPS4A

Plasmid

#169319

Purpose

Gateway entry vector for human VPS4A ORF

Depositor

Francisca Vazquez

Article

Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

Insert

VPS4A (VPS4A Human)

Use

Gateway entry vector

Promoter

NA

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP6_nostop

Plasmid

#149309

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Bassoon scFv [L124/59]

Plasmid

#182078

Purpose

Mammalian Expression of Bassoon scFv. Derived from hybridoma L124/59.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Bassoon (Rattus norvegicus) (Bsn Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-PTPR [N165/43R]

Plasmid

#188219

Purpose

Mammalian Expression Plasmid of anti-Pan-PTPR. Derived from hybridoma N165/43

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-PTPR recombinant mouse monoclonal antibody (PTPRF Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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KHC068 BRD2-KI-GFP-2A-L387A

Plasmid

#231713

Purpose

CRISPR mediated KI of BromoTag at N-terminus of BRD2-GFP-P2A-BromoTag, works well with KHC064 and KHC065

Depositor

Alessio Ciulli

Article

Bond et al J Med Chem. 2021 Oct 28;64(20):15477-155

Insert

Brd2 (BRD2 Human)

Use

CRISPR

Tags

BromoTag and GFP

Expression

Mammalian

Mutation

L387A

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-SMO

Plasmid

#116792

Purpose

Lentiviral expression of SMO

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SMO (SMO Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PDGFRA-Y288C

Plasmid

#116453

Purpose

Lentiviral expression of PDGFRA Y288C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PDGFRA (PDGFRA Human)

Use

Lentiviral

Mutation

Y288C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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