We narrowed to 23,588 results for: CHI

1. pCAT003

   Plasmid

   #171628

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and EF1-a

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXs-IP spGFP-ERGIC53

   Plasmid

   #38270

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Autophagy. 2010 Aug;6(6):764-76.

   Insert

   ERGIC-53 (LMAN1 Human)
   

   Use

   Retroviral

   Tags

   EGFP with Calreticulin signal peptide at N-termin…

   Expression

   Mammalian

   Mutation

   deleted signal peptide (1-30 amino acids)

   Promoter

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpCTRE-CD4

   Plasmid

   #114010

   Purpose

   Dox-inducible Cas9 expression with a CD4 selection marker

   Depositor

   Charles Rudin

   Article

   Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mTurquoise2-Paxillin

   Plasmid

   #176107

   Purpose

   Focal Adhesion marker

   Depositor

   Dorus Gadella

   Article

   Arts et al Front Immunol. 2021 May 18;12:667213. do

   Insert

   Paxillin (PXN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-GfaABC1D-ERCreER-4x6T

   Plasmid

   #196411

   Purpose

   Astrocytic expression of ERT2-Cre-ERT2 recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   ERT2-Cre-ERT2
   

   Use

   AAV; Astrocyte-selective

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and GfaABC1D

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag

   Plasmid

   #132407

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dLwaCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   R474A; H479A; R1046A; R1051A

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pC001 - huLshC2C2-MBP for bacterial expression

   Plasmid

   #79150

   Purpose

   Expresses human codon-optimized LshC2c2 for purification in E. coli

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   C2c2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR CD19 tandem CAR

   Plasmid

   #113019

   Purpose

   for lentivirus production of CD19 tandem CAR

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   CD19 CAR-P tandem FcR gamma intracellular and PI3k recruiting domain from CD19 (CD19 Mouse, Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAWPSG-Ptet-nCas9-BE

   Plasmid

   #195739

   Purpose

   Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet(Tetracycline inducible protein)

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TBX5-FLAG in pLX303

    Plasmid

    #42563

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    TBX5 (TBX5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRRLSIN.cPPT.PGK-GFP-PTS1.WPRE

    Plasmid

    #133282

    Purpose

    Lentiviral expression of a peroxisomal eGFP

    Depositor

    Etienne Sokal

    Article

    Demaret et al Histochem Cell Biol. 2020 Mar 2. pii: 10

    Insert

    Peroxisome targeting signal 1
    

    Use

    Lentiviral

    Tags

    Peroxisome targeting signal 1

    Expression

    Mammalian

    Mutation

    Promoter

    None

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDARMO_CMVT_FLAG-HUWE1

    Plasmid

    #187121

    Purpose

    mammalian cell expression of FLAG-HUWE1

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 6599-SUMO1GG

    Plasmid

    #13377

    Depositor

    Anne Dejean

    Article

    Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mature form ending with GG

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDarmo.CMVT_v1

    Plasmid

    #133072

    Purpose

    Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-Flag-TwinStrep and TwinStrep-Flag-3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMVT (Enhanced CMV)

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKD001 pME minprom_EGFP

    Plasmid

    #195955

    Purpose

    Gateway pME fluorophore

    Depositor

    Christian Mosimann

    Article

    Prummel et al Nat Commun. 2019 Aug 26;10(1):3857. doi:

    Insert

    mouse beta-globin minimal promoter EGFP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SNX29-PX (269-391)

    Plasmid

    #119108

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX29-PX (269-391)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST40-MCU-V5-HIS

    Plasmid

    #31731

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19. ():.

    Insert

    MCU (MCU Human)
    

    Use

    Tags

    V5-HIS6X

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. shTBX5 # 2

    Plasmid

    #42550

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTBX5 (TBX5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

19. shTBX5 # 1

    Plasmid

    #42549

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTBX5 (TBX5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pET29a-hNABP1

    Plasmid

    #128306

    Purpose

    Express hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA-HA-MST2

    Plasmid

    #33098

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    MST2 (STK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pME-DreERT2 (JDW 1344)

    Plasmid

    #224523

    Purpose

    A Gateway-compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    DreERT2
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLKO.1-GSK3β-#2

    Plasmid

    #32497

    Depositor

    Alex Toker

    Article

    Yoeli-Lerner et al Mol Cancer Res. 2009 Mar;7(3):425-32. Ep

    Insert

    GSK3B (GSK3B Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pAAVTRE3_tdTomato

    Plasmid

    #135178

    Purpose

    AAV expression of TRE(TRE3G)-driven, tdTomato expression for fluorescent labling

    Depositor

    Tetsuo Yamamori

    Article

    Abe et al Front Neuroanat. 2018 Oct 30;12:89. doi:

    Inserts

    tdTomato
    TRE3G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    a variant of DsRed

    Promoter

    TRE3G

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p5E-dr-myl7-pro (JDW 1148)

    Plasmid

    #229836

    Purpose

    A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    cmlc2/myl7
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV-CAG-flex-MCS--4x6T

    Plasmid

    #196420

    Purpose

    Empty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

    Plasmid

    #218513

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    deletion of the Plac_mrfp cassette

    Promoter

    PgyrA_ermBL-ermB'

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1+DPP6

    Plasmid

    #117272

    Purpose

    Encodes human DPP6 variant 1

    Depositor

    Mark Cannell

    Article

    Lainez et al Biochem Pharmacol. 2018 Apr;150:120-130.

    Insert

    DPP6 (DPP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDEST_FLAG-HUWE1_C4341S

    Plasmid

    #187156

    Purpose

    mammalian cell expression FLAG-HUWE C4341S

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    C4341S

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SNX10-PX (1-201)

    Plasmid

    #119091

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX10-PX (1-201) (SNX10 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHAGE2 Lnp-mCherry

    Plasmid

    #86687

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    LNPK (LNPK Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. MET7A-CD4d3+4-bio

    Plasmid

    #73127

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding MET7A with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    MET7A (METTL7A Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAS_4xMx PylT_GFP 150TAG

    Plasmid

    #140016

    Purpose

    Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG, K46E (Please see depositor comments below)

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLYS6-METTL17-FLAG

    Plasmid

    #220875

    Purpose

    Expressing METTL17-FLAG

    Depositor

    Vamsi Mootha

    Article

    Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

    Insert

    METTL17 (METTL17 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBAD-Padron2

    Plasmid

    #172355

    Purpose

    Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

    Depositor

    Stefan Jakobs

    Article

    Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

    Insert

    Padron2
    

    Use

    Tags

    6xHisTag (N terminal on backbone)

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDEST47-MCU-GFP

    Plasmid

    #31732

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19. ():.

    Insert

    MCU (MCU Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEC3109 (pSpy1C-Plac(1)_ffluc)

    Plasmid

    #218514

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    deletion of mrfp cassette, mutagenesis of the RBS…

    Promoter

    Plac(1)

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid

    #218778

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    deletion of mrfp cassette, mutagenesis of the RBS…

    Promoter

    Plac(3)

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAS_FLAG-Mx1201 PylRS

    Plasmid

    #154764

    Purpose

    plasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pINDUCER20_mStrawberry

    Plasmid

    #161734

    Purpose

    Doxycycline-inducible expression of mStrawberry

    Depositor

    Alec Kimmelman

    Article

    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMTL3

    Plasmid

    #127966

    Purpose

    constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    AAVS1-CAG-KRAB-dCas9-BFP-KRAB
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAAV-Ef1a-DIO mScarlet

    Plasmid

    #131002

    Purpose

    Double floxed mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV5

    Insert

    mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMTL5

    Plasmid

    #127967

    Purpose

    DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221075

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. pMK395 (AAVS1 CMV-OsTIR1(F74A))

    Plasmid

    #159992

    Purpose

    Expression of OsTIR1(F74A) in human cells

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-OsTIR1(F74A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

    Plasmid

    #224489

    Purpose

    Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Sun1-2xsfGFP-6xMYC-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. PEmax_2star_IVT

    Plasmid

    #227674

    Purpose

    plasmid for PEmax** mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    PEmax_2_star
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pOTTC1329 - pAAV EF1a HA-hM4D(Gi)

    Plasmid

    #89150

    Purpose

    An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pSF-p15A-YFP

    Plasmid

    #186563

    Purpose

    p15A ori, promotorless, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

    Depositor

    Teresa de Diego Puente

    Article

    Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

    Insert

    YFP
    

    Use

    Tags

    None

    Expression

    Bacterial and Mammalian

    Mutation

    None

    Promoter

    Promotorless

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pET15b CnA CnB

    Plasmid

    #11787

    Depositor

    Anjana Rao

    Article

    Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

    Insert

    CnA CnB (PPP3R1, PPP3CA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 4, 2006

    Availability

    Academic Institutions and Nonprofits only