We narrowed to 16,007 results for: grn

1. pCMV enAsCas12f-YHAM-sgRNA_v7

   Plasmid

   #208356

   Purpose

   Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

   Depositor

   Atsushi Hoshino

   Article

   Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

   Insert

   enAsCas12f-YHAM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F48Y/S188H/V232A/E316M

   Promoter

   CMV

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

   Plasmid

   #67980

   Purpose

   Cas9 activity reporter with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. FASTHDR-tRNApromo-sgRNA-espCas91_1

   Plasmid

   #167210

   Purpose

   Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 7b sgRNA for EJ7-GFP reporter

   Plasmid

   #113624

   Purpose

   sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7b sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

5. AAV-U6-sgRNA-hSyn-mCherry

   Plasmid

   #87916

   Purpose

   sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

   Depositor

   Alex Hewitt

   Article

   Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

   Plasmid

   #67991

   Purpose

   CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MiniCoopR 2xU6:gRNA, mitfa:Cas9

   Plasmid

   #118844

   Purpose

   Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Type

   Empty backbone

   Use

   CRISPR; Tol2

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-U6-sasgRNACMV- mCherry-WPREpA

   Plasmid

   #217015

   Purpose

   S. aureus sgRNAs backbone along with mCherry expression

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVi U6_gRNA CMV_SadCas9-KRAB

   Plasmid

   #214609

   Purpose

   All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

   Depositor

   Ralf Gilsbach

   Article

   Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

   Inserts

   gRNA
   SadCas9
   

   Use

   AAV

   Tags

   NP NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   CMV and U6

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

    Plasmid

    #67979

    Purpose

    Cas9 activity reporter (control) with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. p426-SNR52p-gRNA.CAN1.Y-SUP4t

    Plasmid

    #43803

    Purpose

    Encodes a gRNA that targets Can1.Y in yeast.

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    CAN1.y gRNA (CAN1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    March 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

    Plasmid

    #67986

    Purpose

    Cas9 activity reporter with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ14107 fwYellow-targeting sgRNA

    Plasmid

    #239456

    Purpose

    guide for fwYellow

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    guide targeting fwYellow
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ14112 tyrosinase-targeting sgRNA

    Plasmid

    #239751

    Purpose

    guide for tyrosinase

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    guide targeting tyrosinase
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-Sp-pegRNA-RNF2_+5GtoT

    Plasmid

    #135957

    Purpose

    S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    RNF2_5GtoT pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-sgRNA(lib)-puro

    Plasmid

    #119976

    Purpose

    Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Wensheng Wei

    Article

    Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

    Plasmid

    #112925

    Purpose

    Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6 promoter, gRNA-SAM expression cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. HH-gRNA-HDV-Shuttle-Vector

    Plasmid

    #169098

    Purpose

    Backbone plasmid to generate HH-gRNA-HDV segment

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Development. 2021 Mar 9. pii: dev.193565

    Insert

    HH-gRNAbackbone-HDV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    NA

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-U6-dgRNA-CAG-MPH

    Plasmid

    #106259

    Purpose

    Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Insert

    MS2-P65-HSF1 (HSF1 Synthetic, Human)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti sgRNA(MS2) mCherry

    Plasmid

    #171053

    Purpose

    Expression of Vp64-dCas9 activation system

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    sgRNA(MS2) mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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