We narrowed to 26,215 results for: grn

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PX459-sgRNA047_Podxl

Plasmid

#232932

Purpose

Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9-T2A-PuroR (Podxl S. pyogenes)

Expression

Mammalian

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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EHMT1_exon 3_gRNA

Plasmid

#228809

Purpose

CRISPR targeting of human EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

gRNA targeting EHMT1 exon 3 (EHMT1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_NbATML1-1pro

Plasmid

#231151

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting NbATML1-1pro

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_NbSTM-5'UTR

Plasmid

#231150

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting NbSTM-5?UTR

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting NbSTM-5?UTR

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-2_M13ps

Plasmid

#231412

Purpose

M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-2

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_LacI_pLac-sgRNA-1

Plasmid

#231408

Purpose

sgRNA-1 expression from an IPTG-inducible promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

sgRNA-1
LacI

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_AraC_pBAD-sgRNA-1

Plasmid

#231407

Purpose

sgRNA-1 expression from an arabinose-inducible promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

sgRNA-1
AraC

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A1_M13ps

Plasmid

#231414

Purpose

M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-A1

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-1_M13ps

Plasmid

#231409

Purpose

M13 phagemid that expresses sgRNA-1 from a strong constitutive promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-1

Use

Synthetic Biology

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF1

Plasmid

#216537

Purpose

gRNA and template for MET 5'UTR uORF1 mutation

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF1 mutation

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF2

Plasmid

#216538

Purpose

gRNA and template for MET 5'UTR uORF2 mutation

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF2 mutation

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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RhoA gRNA #1

Plasmid

#198718

Purpose

Cas9-mediated knockout of RhoA in mammalian cells #1

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoA (Rhoa Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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RhoA gRNA #3

Plasmid

#198720

Purpose

Cas9-mediated knockout of RhoA in mammalian cells #3

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoA (Rhoa Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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RhoB gRNA #1

Plasmid

#198721

Purpose

Cas9-mediated knockout of RhoB in mammalian cells #1

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoB (Rhob Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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RhoB gRNA #2

Plasmid

#198722

Purpose

Cas9-mediated knockout of RhoB in mammalian cells #2

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoB (Rhob Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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RhoB gRNA #3

Plasmid

#198723

Purpose

Cas9-mediated knockout of RhoB in mammalian cells #3

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoB (Rhob Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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RhoC gRNA #1

Plasmid

#198724

Purpose

Cas9-mediated knockout of RhoC in mammalian cells #1

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoC (Rhoc Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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