We narrowed to 14,218 results for: trac

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alpha actinin delta ABD-GFP

Plasmid

#66935

Purpose

Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

Depositor

Pere Roca-Cusachs, Michael Sheetz

Article

Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Alpha-actinin 1 (ACTN1 Human)

Tags

GFP

Expression

Mammalian

Mutation

actin binding domain (amino acids 30–253) is dele…

Promoter

CMV

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

Plasmid

#201819

Purpose

A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter

Depositor

Christopher Richie

Article

Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

Insert

hChR2(H134R)-V5-ER-LBD (ChRERa)

Expression

Mammalian

Promoter

pOTTC1751

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FGFR2-Fv-Fvls-E

Plasmid

#15286

Depositor

David Spencer

Article

Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

Insert

FGFR2 kinase, FKBP12v36 (Fgfr2 Mouse)

Tags

Myristoylation-targeting domain c-Src (14aa) and …

Expression

Mammalian

Mutation

FGFR2 cytoplasmic domain FKBP12 F36V

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain

Plasmid

#187276

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Mutation

deltaActin binding domain

Promoter

U6, CMV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain

Plasmid

#187275

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Mutation

deltaMyosin binding domain

Promoter

U6, CMV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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His-PHD2-181-426

Plasmid

#223551

Purpose

His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-180.

Promoter

trc

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL 4K>R

Plasmid

#202420

Purpose

Expression of ubiquitination-deficient GFP-tagged PODXL (4 cytoplasmic lysines mutated to arginine)

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Mutation

K431/525/526/547R

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLENTI_Hu_ECT2(WT)

Plasmid

#136330

Purpose

Lentiviral expression of human ECT2 WT

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ECT2 (ECT2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FGFR3-Fv-Fvls-E

Plasmid

#15287

Depositor

David Spencer

Article

Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

Insert

FGFR3 kinase, FKBP12v36 (Fgfr3 Mouse)

Tags

Myristoylation-targeting domain c-Src (14aa) and …

Expression

Mammalian

Mutation

FGFR3 cytoplasmic domain only FKBP12 F36V

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx32-IRES-GCaMP6s

Plasmid

#216795

Purpose

Inducible bicistronic lentiviral plasmid for the simultaneous expression of Connexin 32 and cytosolic GCaMP6s

Depositor

Fabio Mammano

Article

Tettey-Matey et al Cell Commun Signal. 2024 Dec 5;22(1):589

Insert

Connexin 32-IRES-GCaMP6s (GJB1 Human)

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pJHG003

Plasmid

#126454

Purpose

Expresses Cas9-XTEN-dTdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-XTEN-dTdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Mutation

Added D343E and D345E to inactivate TdT.

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

Plasmid

#24419

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

RS1 (HTR4 Human)

Use

Mouse Targeting

Tags

FLAG and mCherry

Mutation

Human 5HT4B receptor with D100A mutation, generat…

Available Since

March 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG membrane-bound lipin-1 (TMD)

Plasmid

#120277

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Tags

FLAG and in-frame fusion with the TMD Golgi-local…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-AnillinA740D, E758K

Plasmid

#187273

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Mutation

Anillin A740D, E758K

Promoter

U6, CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-PLXNB2-shRNA2

Plasmid

#98400

Purpose

Lentivirus for expression of shRNA2 against human PLEXIN-B2 (Dox-inducible)

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

Lentiviral

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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RCAS-Neu HA SIINFEKL

Plasmid

#125832

Purpose

Retroviral expression of rat Neu cDNA

Depositor

Yi Li

Article

Du et al Proc Natl Acad Sci U S A. 2006 Nov 14;10

Insert

Neu (Erbb2 Rat)

Use

Retroviral

Tags

2x HA and SIINFEKL

Expression

Mammalian

Mutation

Val to Glu point mutation of codon 664 and trunca…

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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SKM2 X057_pECIA2

Plasmid

#115114

Purpose

Bait vector SKM2 X057_pECIA2 should be used with prey vector SKM2 X057_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G56040 (AT5G56040 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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BAM1 X060_pECIA2

Plasmid

#115117

Purpose

Bait vector BAM1 X060_pECIA2 should be used with prey vector BAM1 X060_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G65700 (BAM1 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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PXC3 X011_pECIA2

Plasmid

#115118

Purpose

Bait vector PXC3 X011_pECIA2 should be used with prey vector PXC3 X011_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G41820 (AT2G41820 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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EFR D5_pECIA2

Plasmid

#115119

Purpose

Bait vector EFR D5_pECIA2 should be used with prey vector EFR D5_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G20480 (EFR Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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