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PacrAB-acrAB-rfp + PmutS-mutS-yfp

Plasmid

#121443

Purpose

plasmid expressing translational fusions of AcrAB and MutS

Depositor

Mary Dunlop

Publication

El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

Inserts

PmutS-mutS-yfp
PacrAB-acrAB-rfp

Use

Synthetic Biology; Pbb vector

Expression

Bacterial

Promoter

PacrAB and PmutS

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

Plasmid

#116885

Purpose

FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

Depositor

Brian Kuhlman

Publication

Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

Insert

LINXa4(RVH)-Bre1

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

Promoter

ADH1

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mYFP

Plasmid

#105144

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P81nmt1-mYFP

Tags

81nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mYFP

Plasmid

#105141

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P41nmt1-mYFP

Tags

41nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pVENUS-N1-FNTA

Plasmid

#86859

Purpose

To express FNTA with a Venus tag at C terminus.

Depositor

Yihong Ye

Publication

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

FNTA (FNTA Human)

Tags

Venus

Expression

Mammalian

Mutation

The sequence of the insert is isoform 2, which di…

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pSGE_763_4xUAS_UPS_hkCP

Plasmid

#71171

Purpose

Luciferase reporter

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

763_4xUAS_UPS_hkCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pSGE_764_4xUAS_DOS_hkCP

Plasmid

#71172

Purpose

Luciferase reporter

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

764_4xUAS_DOS_hkCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_MC19_Trl-2xUAS_dCP

Plasmid

#71170

Purpose

Luciferase reporter

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MC19_Trl-2xUAS_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1517

Plasmid

#62387

Purpose

pFA6a Osm1(M33A)-yoEGFP::LEU2

Depositor

Jonathan Weissman

Publication

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Mutation

Methionine 33 mutated to Alanine

Promoter

Adh1p

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pFP122

Plasmid

#21669

Depositor

James Haber

Publication

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pFP140

Plasmid

#21672

Depositor

James Haber

Publication

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pFP120

Plasmid

#21673

Depositor

James Haber

Publication

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

LacZ (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, a 918…

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pLIA EnR (CC#29)

Plasmid

#15152

Depositor

Connie Cepko

Publication

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

Engrailed repressor (en Fly)

Use

Retroviral

Expression

Mammalian

Mutation

engrailed repressor domain

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pLIA CRX EnR (CC#145)

Plasmid

#15155

Depositor

Connie Cepko

Publication

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

CRX (Crx Mouse)

Use

Retroviral

Tags

Engrailed Repressor

Expression

Mammalian

Mutation

amino acids 7–108, containing the CRX homeobox

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pAS169

Plasmid

#194492

Purpose

tCYC1 (Part 4)

Depositor

Ahmad Khalil

Publication

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

tCYC1 (Part 4)

Use

Synthetic Biology

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAS157

Plasmid

#194480

Purpose

synGR Part (Part 3)

Depositor

Ahmad Khalil

Publication

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synGR (Part 3)

Use

Synthetic Biology

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAS155

Plasmid

#194478

Purpose

synDHBR Part (Part 3)

Depositor

Ahmad Khalil

Publication

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synDHBR (Part 3)

Use

Synthetic Biology

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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CRBN_ΔHBD

Plasmid

#232779

Purpose

Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain

Depositor

Ivan Dikic

Publication

Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2

Insert

Cereblon (CRBN Human)

Tags

MBP-His6-TEV

Expression

Bacterial

Mutation

CRBN (residues 47 to 193 and 249 to 436) with GNG…

Promoter

T7

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

Plasmid

#213973

Purpose

AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

Depositor

Zhonghua Lu

Publication

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-rDA2m

Plasmid

#208700

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m in neurons

Depositor

Yulong Li

Publication

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA2m

Use

AAV

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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