We narrowed to 13,710 results for: crispr cas9 plasmids

1. pTHAP12-N1.1.2-gDNA

   Plasmid

   #175860

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   THAP12-Nickase2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCHCHD3-N2.1.2-gDNA

   Plasmid

   #175858

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   CHCHD3-Nickase2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCHCHD3-N1.1.1-gDNA

   Plasmid

   #175857

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   CHCHD3-Nickase1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZNF668.1.0-gDNA

   Plasmid

   #132463

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   ZNF668 (ZNF668 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCENPBD1.1.0-gDNA

   Plasmid

   #132460

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   CENPBD1 (CENPBD1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTHAP7.1.0-gDNA

   Plasmid

   #132439

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   THAP7 (THAP7 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

   Plasmid

   #68357

   Purpose

   The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Inserts

   5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
   FlpO recombinase
   

   Use

   CRISPR

   Tags

   mTurquoise2 (BFP)

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and synthetic Probasin ARRx2

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. HCP9

   Plasmid

   #166111

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2

   Depositor

   Matthias Heinemann

   Insert

   Hta2-sg18 (HTA2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-U6-sgRNA-CMV-GFP

   Plasmid

   #85451

   Purpose

   Express sgRNA in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pCMV-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pCMV-EGFP

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-entry-puro

    Plasmid

    #85745

    Purpose

    Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. HCP9

    Plasmid

    #166111

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2

    Depositor

    Matthias Heinemann

    Insert

    Hta2-sg18 (HTA2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX458_ATF4_2

    Plasmid

    #72352

    Purpose

    Encodes gRNA for 3' target of human ATF4 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Insert

    ATF4 (ATF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458_ATF4_1

    Plasmid

    #72351

    Purpose

    Encodes gRNA for 3' target of human ATF4 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Insert

    ATF4 (ATF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458_DNMT3B

    Plasmid

    #72366

    Purpose

    Encodes gRNA for 3' target of human DNMT3B along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Insert

    DNMT3B (DNMT3B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. PTG

    Plasmid

    #127198

    Purpose

    Single template U6 promoter minimal size plasmid

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Type

    Empty backbone

    Use

    Empty sgrna plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458_ATF4_2

    Plasmid

    #72352

    Purpose

    Encodes gRNA for 3' target of human ATF4 along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    ATF4 (ATF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX458_ATF4_1

    Plasmid

    #72351

    Purpose

    Encodes gRNA for 3' target of human ATF4 along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    ATF4 (ATF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PX458_DNMT3B

    Plasmid

    #72366

    Purpose

    Encodes gRNA for 3' target of human DNMT3B along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    DNMT3B (DNMT3B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid

    #99692

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHSN6A01

    Plasmid

    #50586

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-VP64
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Expression

    Mutation

    dCas9 = defective in DNA cleavage; 4×minimal VP16…

    Promoter

    2×35Sp and AtU6-26p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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