Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 6,770 results for: hwa

Showing: 4741 - 4760 of 6770 results

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pCMV6-MPP8-delta2-441-10xHis

Plasmid

#194181

Purpose

Expresses MPP8-delta2-441_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta2-441

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta442-860-10xHis

Plasmid

#194182

Purpose

Expresses MPP8-delta442-860_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta442-860

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta600-728-10xHis

Plasmid

#194183

Purpose

Expresses MPP8-delta600-728_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta600-728 (4xANK deletion)

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hCHAT)-PGKpuro2ABFP-W

Plasmid

#163147

Purpose

Lentiviral gRNA plasmid targeting human CHAT gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

CHAT (CHAT Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2ABFP-W

Plasmid

#163175

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2ABFP-W

Plasmid

#163174

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hAP1 1-419

Plasmid

#183644

Purpose

To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

ATP6AP1 (ATP6AP1 Human)

Tags

myc

Expression

Mammalian

Mutation

truncated ATP6AP1 mutant; contains only amino aci…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hAP1 Δ420-440

Plasmid

#183642

Purpose

To (stably) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; lentiviral expression

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

ATP6AP1 (ATP6AP1 Human)

Use

Lentiviral

Tags

HA

Mutation

truncated ATP6AP1 mutant lacking the transmembran…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hSEC61B-C-PEN2

Plasmid

#183648

Purpose

Lentiviral expression of a ER-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and SEC61B (PSENEN, SEC61B Human)

Use

Lentiviral

Tags

HA

Mutation

WT

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)

Plasmid

#171099

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Ascl1 S. pyogenes, Mouse)

Use

CRISPR

Promoter

Ef1a

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4(TA)-ires-blast

Plasmid

#167831

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2ABFP-W

Plasmid

#163170

Purpose

Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

YAP1 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2AmCherry-W

Plasmid

#163172

Purpose

Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

YAP1 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 R30W-T2A-GFP

Plasmid

#163786

Purpose

Expression of R30W CARD domain variant

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

R30W

Promoter

MND

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 K41R-T2A-GFP

Plasmid

#163779

Purpose

Expression of K41R CARD domain variant

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

K41R

Promoter

MND

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 R35Q-T2A-GFP

Plasmid

#163774

Purpose

Expression of R35Q CARD domain variant

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

R35Q

Promoter

MND

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 delta e4-T2A-GFP

Plasmid

#163771

Purpose

Expression of CARD domain variant with a deletion of exon 4

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

Del exon 4

Promoter

MND

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 R72Q-T2A-GFP

Plasmid

#163783

Purpose

Expression of R72Q CARD domain variant

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

R72Q

Promoter

MND

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 D58G-T2A-GFP

Plasmid

#163773

Purpose

Expression of D58G CARD domain variant

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

D58G

Promoter

MND

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 delta e3-4-T2A-GFP

Plasmid

#163772

Purpose

Expression of CARD domain variant with a deletion of exons 3 and 4.

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

Del exon 3-4

Promoter

MND

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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