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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384_R

Plasmid

#147384

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384 (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-mut_R

Plasmid

#147385

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-CIM1-mut (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-Nterm6Wmut-SD6Wmut_O

Plasmid

#147154

Purpose

Insect Expression of DmGW182-Nterm6Wmut-SD6mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182-Nterm6Wmut-SD6mut (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_1-1089_K

Plasmid

#146804

Purpose

Mammalian Expression of HsNot1iso1_1-1089

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1-1089 (CNOT1 Human)

Expression

Mammalian

Mutation

Deletion of 5 AA compared to the sequence given b…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_1085-1605_K

Plasmid

#146805

Purpose

Mammalian Expression of HsNot1iso1_1085-1605

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1085-1605 (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1-1089_K

Plasmid

#146806

Purpose

Mammalian Expression of HsNot1iso1_1-1089

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1-1089 (CNOT1 Human)

Expression

Mammalian

Mutation

Deletion of 5 AA compared to the sequence given b…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1085-1605_K

Plasmid

#146807

Purpose

Mammalian Expression of HsNot1iso1_1085-1605

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1085-1605 (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(T304I)

Plasmid

#177133

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_C_FH_VIR

Plasmid

#186660

Purpose

Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Shu

Plasmid

#186655

Purpose

Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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piwi_sgRNA

Plasmid

#186653

Purpose

piwi sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Piwi sgRNA Plasmid (piwi Fly)

Expression

Insect

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Piwi

Plasmid

#186651

Purpose

Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_C_FH_Nbr

Plasmid

#186662

Purpose

Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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Nbr_C_sgRNA2

Plasmid

#186664

Purpose

Nbr C-tag sgRNA2 plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Nbr sgRNA 2 Plasmid (Nbr Fly)

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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Nbr_C_sgRNA1

Plasmid

#186663

Purpose

Nbr C-tag sgRNA1 plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Nbr sgRNA 1 Plasmid (Nbr Fly)

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDonor_N_FH_GLE

Plasmid

#186657

Purpose

Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(K72A)

Plasmid

#177132

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S291D

Plasmid

#115193

Purpose

Lentiviral transduction and expression of PDHA2S291D into any mammalian cell

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S291D (PDHA2 Human)

Use

Lentiviral

Mutation

p.S291D

Promoter

EF1alpha

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S293D

Plasmid

#115194

Purpose

Lentiviral transduction and expression of PDHA2S293D into any mammalian cell

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S293D (PDHA2 Human)

Use

Lentiviral

Mutation

p.S293D

Promoter

EF1alpha

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S291D/S293D

Plasmid

#115195

Purpose

Lentiviral transduction and expression of PDHA2S291D/S293D into any mammalian cell

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S291D/S293D (PDHA2 Human)

Use

Lentiviral

Mutation

p.S291D/S293D

Promoter

EF1alpha

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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