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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 4761 - 4780 of 66674 results

  1. mApple-Ezrin-C-14

    Plasmid
    #54896
    Purpose
    Localization: Focal Adhesions/Membrane, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Ezrin (EZR Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. mApple-Ezrin-N-14

    Plasmid
    #54897
    Purpose
    Localization: Focal Adhesions/Membrane, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Ezrin (EZR Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. FL-BCL2L1

    Plasmid
    #78860
    Purpose
    DULIP positive control (prey) for assay establishment
    Depositor
    Erich Wanker
    Publication
    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BCL2 like 1 (BCL2L1 Human)
    Use
    Expression vector
    Tags
    Firefly luciferase, V5-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    78860-plasmid-map-sequence-id-147489

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  10. pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

    Plasmid
    #135013
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    135013-plasmid-map-sequence-id-267601

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  11. mWasabi-EB3-7

    Plasmid
    #56499
    Purpose
    Localization: MT End Binding Protein, Excitation: 493, Emission: 509
    Depositor
    Michael Davidson
    Insert
    EB3 (MAPRE3 Human)
    Tags
    mWasabi
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. TFORF1125

    Plasmid
    #143093
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ENO1 (ENO1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143093-plasmid-map-sequence-id-399018

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  15. p426_Cas9_gRNA-HIS3b

    Plasmid
    #87402
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting HIS3b sequence AATATAGAGTGTACTAGAGG in yeast chromosome 15.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting HIS3b
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87402-plasmid-map-sequence-id-508301

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  16. p426_Cas9_gRNA-ARS208a

    Plasmid
    #87383
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS208a sequence GTCCGCTAAACAAAAGATCT in yeast chromosome 2.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS208a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87383-plasmid-map-sequence-id-193425

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  17. p426_Cas9_gRNA-ARS416d

    Plasmid
    #87386
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS416d sequence TAGTGCACTTACCCCACGTT in yeast chromosome 4.
    Depositor
    Aindrila Mukhopadhyay
    Publication
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS416d
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87386-plasmid-map-sequence-id-193428

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  20. SARS CoV-2 HexaPro spike (bio-His)

    Plasmid
    #166856
    Purpose
    Human expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.
    Depositor
    Evelien Bunnik
    Publication
    Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. d
    Insert
    SARS CoV-2 HexaPro full length spike protein (S Severe acute respiratory syndrome-related coronavirus)
    Tags
    8xHis and Biotinylation site
    Expression
    Mammalian
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    166856-plasmid-map-sequence-id-327668

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Showing: 4761 - 4780 of 66674 results