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pFAST NPC1(NTD)-3Q

Plasmid

#25772

Depositor

Johann Deisenhofer

Article

Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

Insert

npc1 (NPC1 Human)

Tags

His

Expression

Insect

Mutation

aa23-252 only; N70Q N122Q N185Q

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pPI184

Plasmid

#113221

Purpose

generic knockin vector for C-terminal tagging with mCherry

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

mCherry

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAdTrackCMV-CDKN2A

Plasmid

#37105

Depositor

Joseph Nevins

Article

Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

Insert

CDKN2A (CDKN2A Human)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
1025

Plasmid

#160235

Purpose

FlightN-TetR(OFF)-VP16-Sv40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Tetracycline repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-AR10

Plasmid

#138980

Purpose

Endogenous RAS regulation using light-controlled intrabody

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

BglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDM1018

Plasmid

#108975

Purpose

shuttle vector, N-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

mCherry

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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EB3-eGFP-2xC2-iLID

Plasmid

#114419

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pPI464

Plasmid

#113216

Purpose

generic knockin vector for C-terminal tagging with mScarlet

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

mScarlet

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS_2paCRY2(D387A)

Plasmid

#129652

Purpose

2P-activatable CRY2 with the FAD-disrupting mutation

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2(D387A)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pZLSLD.R

Plasmid

#51511

Purpose

Single component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS

Promoter

2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pDD375

Plasmid

#91825

Purpose

Codon-optimized mKate2 donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mKate2-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-prepore

Plasmid

#160519

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDD397

Plasmid

#91822

Purpose

AID FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pfast NPC1(NTD)

Plasmid

#25773

Depositor

Johann Deisenhofer

Article

Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

Insert

npc1 (NPC1 Human)

Tags

His

Expression

Insect

Mutation

aa 23-252 only

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
bTub-GFP.766A

Plasmid

#112695

Purpose

expressed GFP in testes, under bTub promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

bTub-GFP-SV40

Expression

Insect

Promoter

bTub

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR-I70V

Plasmid

#47663

Purpose

pAC-EsaR with a208g mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-I70V

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed Isoleucine 70 to Valine

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pDM1049

Plasmid

#108992

Purpose

Inducible expression vector (dox-on), C-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Tags

GFP

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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OptoOFF-MxCBD-mFGFR1_623

Plasmid

#105825

Purpose

Expresses OptoOFF-MxCBD-mFGFR1 (light-inactivated receptor tyrosine kinase)

Depositor

Harald Janovjak

Article

Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

Insert

OptoOFF-MxCBD-mFGFR1

Expression

Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-K-I-CAAX

Plasmid

#41642

Purpose

Catalytically active control, intersectin Dbl homology (DH) domain fused to wild-type Dronpa

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

Dronpa145K-IntersectinDH-CAAX

Tags

CAAX box

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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PSuperPuroRetro-1660s(hPot1)

Plasmid

#11136

Depositor

Christopher Counter

Article

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi against Human Protection of Telomeres 1 (control) (POT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only