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Viral Prep#87306-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only
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pCI-MS2V5-PABPC1 MLLEmut M161A/D165K
Plasmid#65810Purposeexpression of PABPC1-MS2 fusion, double mutant.DepositorInsertPABPC1 (PABPC4 Human)
UseTagsMS2 stem loops and V5ExpressionMammalianMutationMLLEmut M161A/D165KPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-16b-10His-TEV-MCES_VACCA_D1_D12
Plasmid#203671PurposeThe D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavageDepositorInsertMCES_VACCA_D1_D12
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
actin CP alpha 2 beta 2
Plasmid#13452DepositorUseTagsExpressionBacterialMutationnonePromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pCRII-BbsI-sgRNAscaffold
Plasmid#159352PurposeExpression vector for a customizable guide RNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
p1242 3x-KB-L
Plasmid#26699DepositorInsert3x MHC I KB element - luciferase (NFKB1 Human, Photinus pyralis)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCF1135
Plasmid#222106PurposeE. coli - Veillonella shuttle plasmid with single selectable markerDepositorTypeEmpty backboneUseE. coli-veillonella shuttle plasmidTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV4-p105
Plasmid#23288DepositorInsertp105 (NFKB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pCF1148
Plasmid#222330PurposeFluorescent reporter plasmid for VeillonellaDepositorInsertbs2 gene with Veillonella parvula SKV38 mdh promoter
UseE. coli-veillonella shuttle plasmidTagsExpressionBacterialMutationPromoterVeillonella parvula SKV38 mdh promoterAvailable sinceOct. 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3-myc- mCE(Del 25C)
Plasmid#82472PurposeExpresses myc tag and mRNA capping enzyme without 25 amino acid from C-terminalDepositorInsertmyc-mCE-C terminal deleted (Rngtt Mouse)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-biotin-myc- mCE
Plasmid#82475PurposeExpresses mRNA capping enzyme containing N terminal biotin and myc tagDepositorInsertBiotin (Rngtt Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSI-Check2-hRluc-NFkB-firefly
Plasmid#106979PurposeLuciferase reporter with internal control for NFkB signal.DepositorInsertNFKB recognition sites (NFKB1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/ TO myc-cCE-bio (cCE-bio)
Plasmid#82473PurposeExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive formDepositorInsertMyc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Mouse, Synthetic)
UseTagsTEV-Biotin and mycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
hCathepsin B
Plasmid#11249PurposeMammalian expression of human cathepsin BDepositorInsertcathepsin B (CTSB Human)
UseTagsC9 (GTETSQVAPA)ExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-Sac-Aβ(M1-42/C18C33)
Plasmid#173759PurposeExpresses the AβC18C33 peptide.DepositorInsertAβ(M1-42/C18C33) (APP Human)
UseTagsExpressionBacterialMutationchanged valine 18 to cysteine; changed glycine 33…PromoterT7Available sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-attL1-Human ABeta-attR5
Plasmid#160436PurposeEntry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombinationDepositorInsertHomo sapiens amyloid beta precursor protein (APP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 CDS A. thaliana PARP2
Plasmid#175810PurposepENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 2 (PARP2, AT4G02390.1) without stop codon for C-terminal epitope tagsDepositorInsertPARP2 (PARP2 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnoneExpressionMutationPromoternoneAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only