We narrowed to 150,108 results for: ins

1. Escherichia coli DH10B-MOB

   Bacterial Strain

   #229395

   Purpose

   Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Bacterial Resistance

   60 ug/ml of dap

   Species

   Escherichia coli

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

2. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

   Plasmid

   #195048

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Expression

   Yeast

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

   Plasmid

   #195047

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Expression

   Yeast

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)

   Plasmid

   #195046

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Expression

   Yeast

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Annexin A7-TEV-TwinStrep

   Plasmid

   #198635

   Purpose

   Annexin A7 fibrillization and lipid binding

   Depositor

   Lalit Deshmukh

   Article

   Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

   Insert

   Annexin A7 (ANXA7 Human)
   

   Tags

   TEV-GS-Dual Strep

   Expression

   Bacterial

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TwinStrepSUMO-pvc10-Cas9

   Plasmid

   #243755

   Purpose

   For affinity purification of Pvc10 fused to Cas9

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

   Insert

   pvc10-Cas9
   

   Expression

   Bacterial

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. TwinStrepSUMO-Ad5-SpyCatcher

   Plasmid

   #243751

   Purpose

   For affinity purification of A5 knob fused to SpyCatcher

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

   Insert

   Ad5-SpyCatcher
   

   Expression

   Bacterial

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABEpuro-ERBB2 A775_G776insYVMA

   Plasmid

   #40982

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   A775_G776insYVMA

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. Proinsulin-NanoLuc in pLX304

   Plasmid

   #62057

   Purpose

   Luminescent reporter of insulin secretion

   Depositor

   David Altshuler

   Article

   Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

   Insert

   Proinsulin-NanoLuc (Ins2 Mouse, Synthetic)
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   Inserted NanoLuc into C-peptide of mouse Ins2 cod…

   Promoter

   CMV

   Available Since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-EGFR-E746_T751delinsA

    Plasmid

    #116246

    Purpose

    Lentiviral expression of EGFR E746_T751delinsA

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Mutation

    E746_T751delinsA

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-INSR-V5/HIS

    Plasmid

    #201980

    Purpose

    expression of human INSR receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    INSR receptor tyrosine kinase (INSR Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis

    Plasmid

    #232344

    Purpose

    Expresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

    Depositor

    Philip Wong

    Article

    Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

    Insert

    HDGF Like 2 (HDGFL2 Human)
    

    Tags

    GGGS linker, 6xHis and Twin-strep, TEV protease s…

    Expression

    Mammalian

    Mutation

    TDP-43 regulated cryptic exon

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTwist-CMV-HDGFL2_WT_TwinstrepTEV_6xHis

    Plasmid

    #232343

    Purpose

    Expresses wild-type human HDGFL2 protein with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

    Depositor

    Philip Wong

    Article

    Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

    Insert

    HDGF like 2 (HDGFL2 Human)
    

    Tags

    GGGS linker, 6xHis and Twin-strep, TEV protease s…

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD24-ALFA-TwinStrep

    Plasmid

    #240248

    Purpose

    Arabinose-inducible plasmid with ALFA and Twin-Strep tags

    Depositor

    Krishna Reddy, Olga Boudker

    Article

    Reddy et al Nat Struct Mol Biol (2025).

    Type

    Empty backbone

    Tags

    10x HIS, ALFA, Thrombin, and Twin-Strep

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXT128_TwinStrep-SUMO-ShCas12k

    Plasmid

    #135524

    Purpose

    Protein expression construct for ShCast12k

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShCas12k
    

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBluescriptII[attB/Ins1]

    Plasmid

    #74100

    Purpose

    "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Phi C31 attb element
    Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
    

    Expression

    Bacterial

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXT130_TwinStrep-SUMO-ShTnsC

    Plasmid

    #135526

    Purpose

    Protein expression construct for ShTnsC

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsC
    

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXT131_TwinStrep-SUMO-ShTniQ

    Plasmid

    #135527

    Purpose

    Protein expression construct for ShTniQ

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTniQ
    

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXT129_TwinStrep-SUMO-ShTnsB

    Plasmid

    #135525

    Purpose

    Protein expression construct for ShTnsB

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB
    

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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