We narrowed to 6,996 results for: nsf
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Plasmid#163676PurposeExpresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD22
Plasmid#163677PurposeExpresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMD24
Plasmid#163678PurposeExpresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
UseTagsGFPExpressionMammalianMutationFor this partial length ST chimera, two restricti…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
F9F17F-GFP
Plasmid#163679PurposeExpresses a TFR mutant with truncated cytosolic tail and TMD in mammalian cellsDepositorInserttransferrin receptor mutant (TFRC Human)
UseTagsGFPExpressionMammalianMutationThis chimera contains 9 amino acids from the cyto…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA3
Plasmid#171001PurposeHiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIV]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA5
Plasmid#171003PurposeHiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI, R537A]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO FAPP2-GLTPH
Plasmid#170739PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertPLEKHA8-GLTPH (PLEKHA8 Human)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO ACD11
Plasmid#170738PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertacd11 (ACD11 Mustard Weed)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim Actin MCH Puro (CAMP)
Plasmid#104446PurposeExpresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromoteractinAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-AtSDT (JBEI-11535)
Plasmid#87934PurposeCo-expression in S.cerevisiae of 4CL5 and AtSDTDepositorUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG RpH-LAMP1-3xFLAG
Plasmid#163018Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorInsertRpH-LAMP1-3xFLAG (Lamp1 Mouse)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG R2pH-LAMP1-3xFLAG
Plasmid#157940Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
UseTags3XFLAG, mCherry, and pHluorinExpressionMammalianMutationPromoterChick beta actin pomoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK FKBP12(F36V)-OGT (Neo)
Plasmid#154294PurposeLentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoterDepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x HA and FKBP12(F36V)ExpressionMammalianMutationPromoterPGKAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-inactive M3nls
Plasmid#157854PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorInsertdCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
UseTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395APromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
EXT1- D164A-pLJC2-3XFLAG
Plasmid#215828PurposeEctopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cellsDepositorInsertExostosin glycosyltransferase 1 Mutant (EXT1 Human)
UseLentiviralTags3X FlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONOR223 RpH-LAMP1-3xFLAG
Plasmid#163019Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorInsertRpH-LAMP1-3xFLAG (Lamp1 Mouse)
UseGateway systemTags3XFLAGExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONOR223 R2pH-LAMP1-3xFLAG
Plasmid#157941Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
UseGateway systemTags3XFLAG, mCherry, and pHluorinExpressionMutationPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT 5N5A
Plasmid#154280PurposeBacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationN322A, N356A, N390A, N424A, N458A mutations; bact…PromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUMO CPTP
Plasmid#170735PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertCPTP (CPTP Human)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His K206E/K534A hTFNG
Plasmid#70133PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobeDepositorInsertN6His K206e/K534A hTFNG (TF Human)
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationchanged Lys206 to a glutamic acid and Lys534 to a…PromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only