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PiggyBac_8mer_pegArray_bb_pos.2

Plasmid

#238543

Purpose

Golden gate acceptor backbone for position 2 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac_8mer_pegArray_bb_pos.3

Plasmid

#238544

Purpose

Golden gate acceptor backbone for position 3 8-mer pegArrays

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pSpyB-Tn7sg

Plasmid

#248694

Purpose

sgRNA expression vector - pBG35 promoter with Tn7sg negative control sgRNA

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Tn7sg

Expression

Bacterial

Promoter

BG35

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pSpyB-Tn7sg

Plasmid

#248694

Purpose

sgRNA expression vector - pBG35 promoter with Tn7sg negative control sgRNA

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Tn7sg

Expression

Bacterial

Promoter

BG35

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pHK101

Plasmid

#235480

Purpose

Repression of gene VIII by Ptet promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII, cI repressor

Expression

Bacterial

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pHK101

Plasmid

#235480

Purpose

Repression of gene VIII by Ptet promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII, cI repressor

Expression

Bacterial

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pHK408

Plasmid

#235458

Purpose

AND gate receiver with bs for sgRNA2 and sgRNA3

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

AND gate

Use

Synthetic Biology

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pHK408

Plasmid

#235458

Purpose

AND gate receiver with bs for sgRNA2 and sgRNA3

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

AND gate

Use

Synthetic Biology

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pKG0618_pPV1-CMV

Plasmid

#239688

Purpose

BsaI Golden Gate asembly plasmid encoding the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

CMV promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKG0618_pPV1-CMV

Plasmid

#239688

Purpose

BsaI Golden Gate asembly plasmid encoding the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

CMV promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKG1179_pPV1-EFS

Plasmid

#239690

Purpose

BsaI Golden Gate assembly plasmid encoding the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

EFS promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKG1179_pPV1-EFS

Plasmid

#239690

Purpose

BsaI Golden Gate assembly plasmid encoding the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

EFS promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG015

Plasmid

#231336

Purpose

BEVA Golden Gate cloning vector; Narrow host range plasmid. L1-p15A-Gm-ELT3; pNDGG021, pOGG009, pNDGG006, pOGG013. NmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG015

Plasmid

#231336

Purpose

BEVA Golden Gate cloning vector; Narrow host range plasmid. L1-p15A-Gm-ELT3; pNDGG021, pOGG009, pNDGG006, pOGG013. NmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CD_bsd

Plasmid

#139945

Purpose

Resistance gene

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

blasticidin-S deaminase

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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CD_nat

Plasmid

#139946

Purpose

Resistance gene

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

nourseothricin acetyltransferase

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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CF_OriT

Plasmid

#139948

Purpose

Origin of transfer for conjugation cargo plasmids

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

OriT

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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EF_tTpEF2

Plasmid

#139951

Purpose

Diatom terminator

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

Thalassiosira pseudonana EF2 terminator

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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CD_bsd

Plasmid

#139945

Purpose

Resistance gene

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

blasticidin-S deaminase

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CD_nat

Plasmid

#139946

Purpose

Resistance gene

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

nourseothricin acetyltransferase

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge