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pEN243-KH217

Plasmid

#224550

Purpose

Cas9-2A-puro and sgRNA targeting the STOP codon mouse Car2

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

Cas9 and sgRNA targeting the STOP codon of mouse CAR2 (Car2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1-Cys pc2332

Plasmid

#201696

Purpose

Expresses of GFP-tagged mTet1-Cys in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1 Cys (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Only GFP-tagged Cysteine Rich Domain (CRD) of Tet1

Promoter

CAG

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-PDE6B-146

Plasmid

#239098

Purpose

pGL3 luciferase vector with 146bp PDE6B promoter (containing NRL response element).

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Insert

PDE6B promoter (PDE6B Human)

Use

Luciferase

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-SUMO-h-cGAS(T321I)

Plasmid

#232294

Purpose

Expression of mutant human cGAS protein (T321I)

Depositor

Thomas Tuschl

Article

Skeldon et al Commun Chem. 2025 Mar 22;8(1):88. doi: 1

Insert

MB21D1 (CGAS )

Mutation

T321I

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1-DSBH pc2335

Plasmid

#201697

Purpose

Expresses of GFP-tagged mTet1-DSBH in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1 DSBH (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1-delCys pc3903

Plasmid

#201701

Purpose

Expresses of GFP-tagged mTet1-delCys in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1 (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the Cysteine rich domain of Tet1

Promoter

CAG

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s-delCys pc3904

Plasmid

#201702

Purpose

Expresses of GFP-tagged mTet1s-delCys in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the Cysteine rich domain of Tet1s

Promoter

CAG

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:YEATS4_CE_pISceI

Plasmid

#223039

Purpose

Express YEATS4 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

YEATS4 (YEATS4 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:LYZ_CE_pISceI

Plasmid

#223038

Purpose

Express LYZ gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

LYZ (LYZ Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSems meGFP-farnesyl

Plasmid

#222949

Purpose

Expression of C-terminal fragment of HRAS responsible for farnesylation tagged with GFP in mammalian cells

Depositor

Jacob Piehler

Article

Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

Insert

C-terminal fragment of HRAS (170-189) (HRAS Human)

Tags

mEGFP

Expression

Mammalian

Mutation

C-terminal fragment of HRAS (170-189)

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-shCdkn1a(#4)

Plasmid

#228913

Purpose

Knockdown of Cdkn1a in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

shCdkn1a (Cdkn1a Mouse)

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRetroQ-GTSE1-acGFP-S91, 262, 454, 724A

Plasmid

#224741

Purpose

Retroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein with S91, 262, 454, 724A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Retroviral

Tags

acGFP

Mutation

Serine 91, 262, 454, 724 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroQ-GTSE1-acGFP-S91, 262, 454, 724D

Plasmid

#224742

Purpose

Retroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein with S91, 262, 454, 724D mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Retroviral

Tags

acGFP

Mutation

Serine 91, 262, 454, 724 Aspartate; Valine 53 Iso…

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pOGS539_GLuc

Plasmid

#226718

Purpose

Plasmid enabling yeast-mediated expression and secretion of Gaussia luciferase (GLuc)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Gaussia Luciferase

Tags

HA tag and Mating factor alpha secretion signal

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_SRGAP2A_GAP(R527L_K566A)

Plasmid

#222617

Purpose

Expresses full length SRGAP2 with R527L, K566A mt in the Rac1-specific GAP domain that abrogates the GTPase activating function of SRGAP2 without locking the GAP-domain in a form bound to Rac1-GDP.

Depositor

Franck Polleux

Article

Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

Insert

SRGAP2 (SRGAP2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Point mutations R527L and K566A (Rac1-specific GA…

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s_K852R pc3914

Plasmid

#201704

Purpose

Expresses of GFP-tagged mTet1s_K852R in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s-K852R (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Lysine 852 mutated to arginine

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s_K852E pc3915

Plasmid

#201705

Purpose

Expresses of GFP-tagged mTet1s_K852E in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s-K852E (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Lysine 852 mutated to glutamate

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5-TEX264-SIM1_mut-cGFP

Plasmid

#220212

Purpose

Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

GFP

Expression

Mammalian

Mutation

p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mOrange/CALR del52

Plasmid

#214768

Purpose

Mammalian expression of human CALR del52

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Insert

human CALR del52 (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mOrange/CALR ins5

Plasmid

#214769

Purpose

Mammalian expression of human CALR ins5

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Insert

human CALR ins5 (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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