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pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

Plasmid

#166133

Purpose

This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

Depositor

Raphael Gaudin

Article

Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

Insert

hU6-gRNA and hH1-gRNA targeting cldn1 gene

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

Plasmid

#166133

Purpose

This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

Depositor

Raphael Gaudin

Article

Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

Insert

hU6-gRNA and hH1-gRNA targeting cldn1 gene

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 P290L BLAST

Plasmid

#164239

Purpose

lentiviral plasmid for GFP-TREX1 P290L

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

P290L point mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 P290L BLAST

Plasmid

#164239

Purpose

lentiviral plasmid for GFP-TREX1 P290L

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

P290L point mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 Y305C BLAST

Plasmid

#164240

Purpose

lentiviral plasmid for GFP-TREX1 Y305C

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Y305C point mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI CMV GFP-TREX1 Y305C BLAST

Plasmid

#164240

Purpose

lentiviral plasmid for GFP-TREX1 Y305C

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Y305C point mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI CMV GFP-TREX1 D272fs BLAST

Plasmid

#164237

Purpose

lentiviral plasmid for GFP-TREX1 D272fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

D272 frame shift mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI CMV GFP-TREX1 D272fs BLAST

Plasmid

#164237

Purpose

lentiviral plasmid for GFP-TREX1 D272fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

D272 frame shift mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI CMV GFP-TREX1 L287fs BLAST

Plasmid

#164238

Purpose

lentiviral plasmid for GFP-TREX1 L287fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

L287 frame shift mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI CMV GFP-TREX1 L287fs BLAST

Plasmid

#164238

Purpose

lentiviral plasmid for GFP-TREX1 L287fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

L287 frame shift mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-N17-puro

Plasmid

#156169

Purpose

Lentiviral vector for expression of flag-RAP1B-N17, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-N17-puro

Plasmid

#156169

Purpose

Lentiviral vector for expression of flag-RAP1B-N17, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI CMV GFP-TREX1 309* BLAST

Plasmid

#164242

Purpose

lentiviral plasmid for GFP-TREX1 309*

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deletion of amino acids 310-314

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 309* BLAST

Plasmid

#164242

Purpose

lentiviral plasmid for GFP-TREX1 309*

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deletion of amino acids 310-314

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI CMV GFP-TREX1 G306A BLAST

Plasmid

#164241

Purpose

lentiviral plasmid for GFP-TREX1 G306A

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

G306A point mutation

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI CMV GFP-TREX1 G306A BLAST

Plasmid

#164241

Purpose

lentiviral plasmid for GFP-TREX1 G306A

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

G306A point mutation

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-36.47M-48-3xFLAG

Plasmid

#130680

Purpose

Expresses human SMCHD1 Exon1-36.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-36.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D1523_G1960del

Promoter

CMV

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-36.47M-48-3xFLAG

Plasmid

#130680

Purpose

Expresses human SMCHD1 Exon1-36.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-36.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D1523_G1960del

Promoter

CMV

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-SMCHD1-Exon1-46.47A-48-3xFLAG

Plasmid

#130677

Purpose

Expresses human SMCHD1 Exon1-46.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-46.47A-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

M1961_E1992del

Promoter

CMV

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-SMCHD1-Exon1-46.47A-48-3xFLAG

Plasmid

#130677

Purpose

Expresses human SMCHD1 Exon1-46.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-46.47A-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

M1961_E1992del

Promoter

CMV

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge