Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 10,083 results for: pET

Clear keywords
Showing: 4841 - 4860 of 10083 results

  2. pET28a-Coh-ddFLN4-ELP-HIS-ybbr

    Plasmid
    #153446
    Purpose
    E. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Publication
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.CohE
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153446-plasmid-map-sequence-id-306487

    Enlarge

  4. pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid
    #116956
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Publication
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    116956-plasmid-map-sequence-id-227069

    Enlarge

  5. pET11a asyn Q79N (Q109H) (TAC-->TAT)

    Plasmid
    #105736
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein Q79N (Q109H) (TAC-->TAT) (SNCA Human)
    Expression
    Bacterial
    Mutation
    Q79N/Q109H
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105736-plasmid-map-sequence-id-207269

    Enlarge

  7. pET21-10XHis-GST-HRV-Her1-dL5

    Plasmid
    #73216
    Purpose
    Bacterial expression vector for Z1907-dL5(E52D) affibody fusion protein (AF) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Publication
    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44
    Insert
    10XHis-GST-HRV-Her1-dL5 (EGFR Human, Synthetic, Schistosoma japonicum)
    Tags
    10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73216-plasmid-map-sequence-id-137065

    Enlarge

  8. pET21-10XHis-GST-HRV-dL5-Her1

    Plasmid
    #73215
    Purpose
    Bacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Publication
    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44
    Insert
    EGFR (EGFR Human, Synthetic, Schistosoma japonicum)
    Tags
    10XHis-GST-HRV and The dL5 FAP and Her1 affibody …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73215-plasmid-map-sequence-id-137064

    Enlarge

  9. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A/W276A/I279A/I307A/V310A; deltaLIR1+3+4)

    Plasmid
    #223784
    Purpose
    Expression of recombinant protein for purification
    Depositor
    Sascha Martens
    Publication
    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038
    Insert
    BCL2L13 soluble part (BCL2L13 Human)
    Tags
    EGFP-TEV-GST
    Expression
    Bacterial
    Mutation
    I224A/L227A/W276A/I279A/I307A/V310A; deletion of …
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    223784-plasmid-map-sequence-id-448415

    Enlarge

  12. pCJ200

    Plasmid
    #162673
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C and a sequence Y75-F104 to introduce a 6th…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162673-plasmid-map-sequence-id-321521

    Enlarge

  13. pCJ220

    Plasmid
    #162686
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224W, C529S; codon optimized for…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162686-plasmid-map-sequence-id-321468

    Enlarge

  14. pCJ221

    Plasmid
    #162687
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224H, C529F; codon optimized for…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162687-plasmid-map-sequence-id-321457

    Enlarge

  16. pET28a(+)-LMNA-ThrHis-Cter

    Plasmid
    #256366
    Purpose
    Human full length lamin A (LMNA) with C-terminal 6x-His tag and cleavable thrombin side. For expression in E. coli ArcticExpress at 24 h, 11-15°C.
    Depositor
    Andrei Lupas
    Insert
    LMNA (LMNA Human)
    Tags
    His-tag: C-terminal which can be cleaved with thr…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    256366-plasmid-map-sequence-id-523675

    Enlarge

  17. pET28a(+)-LMNA-His-Cter

    Plasmid
    #256367
    Purpose
    Human full length lamin A (LMNA) with C-terminal 6x-His tag WITHOUT thrombin cleavage site. For expression in E. coli ArcticExpress at 24 h, 11-15°C.
    Depositor
    Andrei Lupas
    Insert
    LMNA (LMNA Human)
    Tags
    His-tag: C-terminal which CANNOT be cleaved with …
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    256367-plasmid-map-sequence-id-523676

    Enlarge

  20. pET28-MHL_IFIH1:306-1017

    Plasmid
    #247536
    Purpose
    E. coli expression for protein production. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    IFIH1 (IFIH1 Human)
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    VAR_021594 (H843R) and VAR_021595 (A946T) from te…
    Promoter
    T7
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    247536-plasmid-map-sequence-id-515932

    Enlarge

Showing: 4841 - 4860 of 10083 results