We narrowed to 29,181 results for: pET

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pET-24a-STE2

Plasmid

#133430

Purpose

Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.

Depositor

Gerhard Wagner

Insert

STE2 (STE2 Budding Yeast)

Expression

Bacterial

Mutation

Both native Cys (C59S/C252A) have been mutated. S…

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pet 3d alpha 1 beta 2 CP

Plasmid

#13450

Depositor

John Cooper

Article

Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Insert

capping protein alpha 1 beta 2 (mouse) (Capza1 Mouse)

Expression

Bacterial

Mutation

none

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pET28a-PKM2-G415R

Plasmid

#122693

Purpose

Express His-tagged PKM2 (G415R) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

G415R

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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CNNM3-CBS-YPet

Plasmid

#149686

Purpose

For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

Depositor

Motoyuki Hattori

Article

Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

Insert

CNNM3-CBS-YPet (CNNM3 Human, Synthetic)

Tags

8 histidine tag

Expression

Bacterial

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-Beclin1 (1-242)

Plasmid

#24401

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 1-242 only.

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pET-2HMG-mCherry

Plasmid

#112739

Purpose

Express 2HMG-mCherry in E. coli

Depositor

Kyubong Jo

Article

Lee et al RSC Adv, 2016, 6, 46291-46298

Insert

2HMG-mCherry

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a- hPTP1e-PDZ2

Plasmid

#20377

Depositor

Andrew Lee

Article

Hu et al Biochemistry. 2003 Dec 2. 42(47):13856-6

Insert

hPTP1e PDZ2 (PTPN13 Human)

Expression

Bacterial

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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pET51b-NAD-Luc

Plasmid

#135627

Purpose

Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V0)

Depositor

Kai Johnsson

Article

Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

Insert

NAD-Luc sensor

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 P186C

Plasmid

#66173

Purpose

Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Mutation

P186C

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-MRPP3-His

Plasmid

#67866

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

Depositor

Walter Rossmanith

Article

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP3 (KIAA0391 Human)

Tags

His

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pET28b(+)-SPN+IFS

Plasmid

#83372

Purpose

Expresses SPN and IFS complex in E. coli cell

Depositor

SeWon Suh

Article

Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

Inserts

SPN
IFS

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (1-73) (72P)

Plasmid

#62083

Purpose

Expression of human p53 (1-73) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72P

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pET20b-GpNLuc-6xHis

Plasmid

#135932

Purpose

Expresses the GpNLuc LumiFluor in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

GpNLuc LumiFluor

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET32a-Trx-PP-hNF2

Plasmid

#107148

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

Insert

neurofibromin 2 (NF2 Human)

Tags

(His)6-Thioredoxin

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET-24a-CXCR4

Plasmid

#133431

Purpose

Human CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.

Depositor

Gerhard Wagner

Insert

CXCR4 (CXCR4 Human)

Expression

Bacterial

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2

Plasmid

#116953

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET-PesaR-Clux

Plasmid

#47658

Purpose

PesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

PesaR-C

Use

Synthetic Biology

Expression

Bacterial

Mutation

Additional esa box between -35 site and the origi…

Promoter

PesaR-C

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1delWGR-L713F

Plasmid

#173941

Purpose

Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delWGR-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 521-660; L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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HOXD9 (human) pET

Plasmid

#8570

Depositor

Corey Largman

Insert

HOXD9 (HOXD9 Human)

Tags

T7

Expression

Bacterial

Mutation

PCR human cDNA clone; makes protein by in vitro T…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-27

Plasmid

#172752

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-27

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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