We narrowed to 10,306 results for: ESP

1. pPD_Psra-6::ChR2(H134R)::mCherry

   Plasmid

   #117419

   Purpose

   For selectively expressing ChR2(H134R) in ASH sensory neurons under the expression of a Psra-6 promoter

   Depositor

   Shohei Mitani

   Article

   Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

   Insert

   channel rhodopsin-2 with H134R mutation
   

   Use

   Tags

   Expression

   Worm

   Mutation

   H134R

   Promoter

   sra-6

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBW-m4136464

   Plasmid

   #149534

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCXLE-dCas9VPH-T2A-GFP-shP53

   Plasmid

   #102895

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9VPH-T2A-GFP-shP53
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHis-zfCSF3b

   Plasmid

   #65613

   Purpose

   express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli

   Depositor

   Petr Bartunek

   Article

   Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

   Insert

   Csf3b (csf3b Zebrafish)
   

   Use

   Tags

   (His)6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCGN-ELK-1 Ets mut

   Plasmid

   #27157

   Purpose

   For mammalian expression of human Elk-1 with an HA tag and a mutation in the Ets DNA binding domain

   Depositor

   Ron Prywes

   Article

   Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

   Insert

   ELK1 (ELK1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R62E/R65E/Y66D

   Promoter

   Available since

   Dec. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

6. p3XFLAG-MKL1-~100

   Plasmid

   #27176

   Depositor

   Ron Prywes

   Article

   Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

   Insert

   MKL1 (MRTFA Human)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Deletion of 100 aa from ATG

   Promoter

   Available since

   Dec. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pGBW-m4046799

   Plasmid

   #145577

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLKO.1-puro-PARG shRNA

   Plasmid

   #23260

   Depositor

   Robert Sobol

   Article

   Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

   Insert

   poly(ADP-ribose) glycohydrolase shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

   Plasmid

   #191627

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GG-EBNA-EEA-guide1-PGK-Puro

    Plasmid

    #102904

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-guide1-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. SEPTOPO

    Plasmid

    #64944

    Purpose

    source of SEP (Super Ecliptic pHluorin) sequence

    Depositor

    Jeremy Henley

    Article

    Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

    Insert

    SEP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pGBW-m4136461

    Plasmid

    #149538

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pGBW-m4046758

    Plasmid

    #145670

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLHCX-Flag-mPKM2(K433E)

    Plasmid

    #42514

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 Lys433->Glu
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Changed lysine 433 to glutamate

    Promoter

    Available since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-SMVP-Cas9N-U6-gRNA TdL

    Plasmid

    #80938

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJET1.2-U6

    Plasmid

    #173157

    Purpose

    Single guide RNA cassette under U6 promoter

    Depositor

    Piotr Ziolkowski

    Article

    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

    Insert

    sgRNA cassette under U6 promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRVGP-BB

    Plasmid

    #15380

    Depositor

    Stephen Smale

    Article

    Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

    Insert

    mU6-BRG/BRM shRNA (Smarca4, Smarca2 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2008

    Availability

    Academic Institutions and Nonprofits only

18. plko.1/shMCT4 477

    Plasmid

    #99598

    Purpose

    Knockdown of MCT4 expression

    Depositor

    Eli Bar

    Article

    Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

    Insert

    MCT4 (SLC16A3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. -4200GAMT-P1-pGL4

    Plasmid

    #26804

    Depositor

    Sam Lee

    Article

    Ide et al Mol Cell. 2009 Nov 13. 36(3):379-92.

    Insert

    Guanidinoacetate N-methyltransferase (GAMT Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pDendra2-Hygro

    Plasmid

    #202407

    Purpose

    Dendra2 expression plasmid

    Depositor

    Morito Kurata

    Article

    Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

    Insert

    Hygromycin
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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