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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,649 results for: Pol

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Showing: 4861 - 4880 of 7649 results

  3. pCJ197

    Plasmid
    #162670
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    Tags
    His
    Expression
    Bacterial
    Mutation
    S131G; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162670-plasmid-map-sequence-id-321452

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  9. pOCC194

    Plasmid
    #118882
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    Tags
    SNAP tag, cleavable with TEV protease, HIS6, clea…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118882-plasmid-map-sequence-id-232359

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  10. pOCC231

    Plasmid
    #118885
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease and mCitrine…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118885-plasmid-map-sequence-id-232309

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  11. pOCC254

    Plasmid
    #118889
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease; mCherry, cl…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118889-plasmid-map-sequence-id-232286

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  13. MTHFR (MTHFRA-c042)

    Plasmid
    #110271
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MTHFR (MTHFR Human)
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110271-plasmid-map-sequence-id-212270

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  15. pHT-PRD

    Plasmid
    #87755
    Purpose
    Bacterial expression vector for producing 6x histidine tagged dynamin 1a proline rich domain (residues 746-864)
    Depositor
    Yu-Wen Hwang
    Insert
    Semi-synthetic gene coding for dynamin 1a proline rich domain (residues 746-864)
    Tags
    6X histidine tag
    Expression
    Bacterial
    Promoter
    T7 RNA polymerase promoter
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87755-plasmid-map-sequence-id-173677

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  17. pMpGWB425

    Plasmid
    #68690
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Triple repeats of Citrine (3xCitrine)
    Expression
    Plant
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68690-plasmid-map-sequence-id-122052

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Showing: 4861 - 4880 of 7649 results