We narrowed to 6,588 results for: siae

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pDest17_Gcn4 ILVtoED W

Plasmid

#231871

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoAS

Plasmid

#231859

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoAS

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoAS

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED

Plasmid

#231857

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123D, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRS316-SSD1-L1374

Plasmid

#226278

Purpose

Plasmid expressing the SSD1 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SSD1 (SSD1 Budding Yeast)

Expression

Yeast

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAM201

Plasmid

#227660

Purpose

pRS305 3XFLAG-HRV3C-Rpn5-VTENKIF

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

3x-FLAG-HRV3C-

Expression

Yeast

Mutation

(amino acids 121-131) VTENKIF mutated to AAAAAAA

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM72

Plasmid

#227622

Purpose

pETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)

Depositor

Andreas Martin

Article

Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

Inserts

Rpn11 (RPN11 Budding Yeast)
rpn8 (RPN8 Budding Yeast)

Tags

His-Prescission

Expression

Bacterial

Mutation

1-179 and aa 2-239, G77P

Promoter

T7

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM315 pACYC-His6-Rpn10[ΔUIM]

Plasmid

#226348

Purpose

Expression of yeast Rpn10 with the UIM deleted

Depositor

Andreas Martin

Article

Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

Insert

rpn10 (RPN10 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

delta UIM

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-NCYC110

Plasmid

#226273

Purpose

Plasmid expressing the SEC22 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-L1374

Plasmid

#226271

Purpose

Plasmid expressing the SEC22 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-UWOPS872421

Plasmid

#226272

Purpose

Plasmid expressing the SEC22 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-UWOPS872421

Plasmid

#226276

Purpose

Plasmid expressing the SEC18 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-S288C

Plasmid

#226270

Purpose

Plasmid expressing the SEC22 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SSD1-NCYC110

Plasmid

#226279

Purpose

Plasmid expressing the SSD1 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SSD1 (SSD1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pML104-LEU2-2

Plasmid

#226263

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-UWOPS872421

Plasmid

#226268

Purpose

Plasmid expressing the SCT1 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgSeLEU2

Plasmid

#224870

Purpose

Disruption of S. eubayanus type LEU2 genes

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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300_pETcon_SARS2_FLip

Plasmid

#222230

Purpose

yeast surface display of the SARS-CoV-2 FLip variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 FLip RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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299_pETcon_SARS2_EG-5

Plasmid

#222232

Purpose

yeast surface display of the SARS-CoV-2 EG.5 variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 EG.5 RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[Y137A]

Plasmid

#221198

Purpose

ScChd1-aa118-1274 chromatin remodeler with Y137A mutation

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

Y137A

Promoter

T7

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-Gal4-p53

Plasmid

#171076

Purpose

Expresses Gal4-p53 fusion protein in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4 DBD (NEWENTRY Budding Yeast)

Tags

His and p53(1-85)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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