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msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry

Plasmid

#218655

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

Plasmid

#218636

Purpose

Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

H781E, Q782R, F785A

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-SFFVp-gI-BlastR

Plasmid

#207179

Purpose

Gateway compatible Lentiviral Expression Plasmid SFFVp-IRES-BlastR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFVp

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template

Plasmid

#215000

Purpose

Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)

Use

Hdr template

Tags

3xHA

Promoter

None

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-GFP-2A-Puro-gATRIP_A

Plasmid

#211537

Purpose

sgRNA-A against ATRIP

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ATRIP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaD

Plasmid

#210448

Purpose

Express waaD under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaD

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaJ

Plasmid

#210450

Purpose

Express waaJ under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaJ

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaK

Plasmid

#210451

Purpose

Express waaK under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaK

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWaaX

Plasmid

#210459

Purpose

Express waaX under the control of PBAD

Depositor

Sébastien Rodrigue

Article

Allard et al Commun Biol. 2023 Nov 16;6(1):1172. doi:

Insert

waaX

Expression

Bacterial

Promoter

PBAD

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-sp6-kcnk4b

Plasmid

#213123

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk4b (LOC101882857 Zebrafish)

Use

Cloning

Mutation

Stop codon removed

Promoter

T7

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk19/kcnk12l

Plasmid

#209001

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk19 (kcnk12l Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk1a

Plasmid

#208981

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk1a (kcnk1a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk2b

Plasmid

#208984

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk2b (kcnk2b Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk4a

Plasmid

#208987

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk4a (kcnk4a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk5b

Plasmid

#208989

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk5b (kcnk5b Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk10a

Plasmid

#208993

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk10a (kcnk10a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk10b

Plasmid

#208994

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk10b (kcnk10b Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-GFP-2A-Puro-gRAD9A_B

Plasmid

#211612

Purpose

sgRNA-B against RAD9

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA RAD9A

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-GFP-2A-Puro-gRHNO1_B

Plasmid

#211614

Purpose

sgRNA-B against Rhno

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA RHNO1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-GFP-2A-Puro-gRHNO1_A

Plasmid

#211613

Purpose

sgRNA-A against Rhno

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA RHNO1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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