Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,837 results for: pET

Showing: 4881 - 4900 of 9837 results

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pFOX8-Tier1-PhCMV-YPet

Plasmid

#169506

Purpose

Tier-1 vector encoding PhCMV-driven YPet expression (PhCMV-YPet-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven yellow fluorescent protein Ypet

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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3020_pETcon-SARS-CoV-2-RBD_N501Y

Plasmid

#184405

Purpose

yeast surface display of the SARS-CoV-2 Alpha variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Alpha Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

N501Y

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_mouse-Caspase-1-H340D_enzymatic-domain

Plasmid

#183388

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 (AA130-404)

Tags

His-TEV

Expression

Bacterial

Mutation

H340D

Promoter

T7

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_canine-Caspase-1/4_enzymatic-domain

Plasmid

#183383

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4 (AA130-404) (CASP4 Canis lupus familiaris)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmGe1_1220-1354_G

Plasmid

#146420

Purpose

Bacterial Expression of DmGe1_1220-1354

Depositor

Elisa Izaurralde

Article

Jinek et al RNA. 2008 Oct . 14(10):1991-8.

Insert

DmGe1_1220-1354 (Ge-1 Fly)

Expression

Bacterial

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmEDC3_331-440_H

Plasmid

#146448

Purpose

Bacterial Expression of DmEDC3_331-440

Depositor

Elisa Izaurralde

Insert

DmEDC3_331-440 (Edc3 Fly)

Expression

Bacterial

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-30-GST-DmBelle_695-798_B

Plasmid

#145961

Purpose

Bacterial Expression of DmBelle_695-798

Depositor

Elisa Izaurralde

Insert

DmBelle_695-798 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmBelle_1-298_B

Plasmid

#145964

Purpose

Bacterial Expression of DmBelle_1-298

Depositor

Elisa Izaurralde

Insert

DmBelle_1-298 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmBelle_695-798_B

Plasmid

#145965

Purpose

Bacterial Expression of DmBelle_695-798

Depositor

Elisa Izaurralde

Insert

DmBelle_695-798 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmBelle_299-694_B

Plasmid

#145952

Purpose

Bacterial Expression of DmBelle_299-694

Depositor

Elisa Izaurralde

Insert

DmBelle_299-694 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmBelle_299-798_B

Plasmid

#145953

Purpose

Bacterial Expression of DmBelle_299-798

Depositor

Elisa Izaurralde

Insert

DmBelle_299-798 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmBelle_695-798_B

Plasmid

#145954

Purpose

Bacterial Expression of DmBelle_695-798

Depositor

Elisa Izaurralde

Insert

DmBelle_695-798 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-30-GST-DmBelle_1-298_B

Plasmid

#145957

Purpose

Bacterial Expression of DmBelle_1-298

Depositor

Elisa Izaurralde

Insert

DmBelle_1-298 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-30-GST-DmBelle_1-694_B

Plasmid

#145958

Purpose

Bacterial Expression of DmBelle_1-694

Depositor

Elisa Izaurralde

Insert

DmBelle_1-694 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-30-GST-DmBelle_299-694_B

Plasmid

#145959

Purpose

Bacterial Expression of DmBelle_299-694

Depositor

Elisa Izaurralde

Insert

DmBelle_299-694 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-30-GST-DmBelle_299-798_B

Plasmid

#145960

Purpose

Bacterial Expression of DmBelle_299-798

Depositor

Elisa Izaurralde

Insert

DmBelle_299-798 (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-30-GST-DmGW182_1-539_F

Plasmid

#146264

Purpose

Bacterial Expression of DmGW182_1-539

Depositor

Elisa Izaurralde

Insert

DmGW182_1-539 (gw Fly)

Expression

Bacterial

Mutation

one non silent mutation G349A (G117R) compared to…

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (E60amb)-His

Plasmid

#168043

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Glu 60 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop C

Plasmid

#171781

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop C

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Asp1…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-SpyTag003 Loop B

Plasmid

#171777

Purpose

Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-SpyTag003 Loop B

Tags

His6

Expression

Bacterial

Mutation

SpyTag003 and linkers inserted between residues A…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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