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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,887 results for: sequence

Showing: 4881 - 4900 of 12887 results

  2. pOpen-GBDpol (exo)

    Plasmid
    #165572
    Purpose
    Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165572-plasmid-map-sequence-id-340932

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  4. pcDNA3.3_SARS2_BA.2.86

    Plasmid
    #208441
    Purpose
    expressing SARS-CoV-2 BA.2.86 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (BA.2.86) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    see GISAID sequence
    Promoter
    CMV
    Available since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    208441-plasmid-map-sequence-id-410934

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  5. pcDNA3.1-MS2-HA-HsDIS3_Z

    Plasmid
    #148111
    Purpose
    Mammalian Expression of HsDIS3
    Depositor
    Elisa Izaurralde
    Insert
    HsDIS3 (DIS3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L760F compared to the sequence given by isoform 2…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148111-plasmid-map-sequence-id-407051

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  6. Q-IL6-9

    Plasmid
    #166552
    Purpose
    scFv of a human scaffold targeting Interleukin-6. Antigen coverage aa 28-212 of 212, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166552-plasmid-map-sequence-id-328267

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  7. pBJKS-mPar4

    Plasmid
    #53232
    Purpose
    mammalian expression of mouse Par4
    Depositor
    Shaun Coughlin
    Insert
    Par4 (F2rl3 Mouse)
    Use
    Tags
    FLAG and bovine prolactin signal sequence (MDSKGS…
    Expression
    Mammalian
    Mutation
    Deletion of aa1-17 (native signal peptide)
    Promoter
    Available since
    Aug. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pRK GFP Paxillin

    Plasmid
    #50529
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    Paxillin (PXN Human)
    Use
    Tags
    GFP21
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50529-plasmid-map-sequence-id-203086

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  10. T-IL2-12

    Plasmid
    #166564
    Purpose
    scFv of a human scaffold targeting Interleukin-2. Antigen coverage aa 21-153 of 153, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    166564-plasmid-map-sequence-id-327819

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  11. pT7-V5-SBP-C1-HsDIS3_Z

    Plasmid
    #148137
    Purpose
    Mammalian Expression of HsDIS3
    Depositor
    Elisa Izaurralde
    Insert
    HsDIS3 (DIS3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L760F compared to the sequence given by isoform 2…
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148137-plasmid-map-sequence-id-453676

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  12. Q-IL18-6

    Plasmid
    #166554
    Purpose
    scFv of a human scaffold targeting Interleukin-18. Antigen coverage aa 37-193 of 193, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166554-plasmid-map-sequence-id-329244

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  13. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177433
    Purpose
    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.
    Depositor
    David Andrews
    Insert
    ActA tail anchor (actA Listeria monocytogenes)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177433-plasmid-map-sequence-id-348464

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  14. pLNHA-C1-HsNot1iso1-shRNAres_V

    Plasmid
    #147749
    Purpose
    Mammalian Expression of HsNot1iso1-shRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsNot1iso1-shRNAres (CNOT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    1 silent mutation compared to the sequence of iso…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147749-plasmid-map-sequence-id-444359

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  15. pUPD2 PhyB (GB2721)

    Plasmid
    #160603
    Purpose
    chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch.
    Depositor
    Diego Orzaez
    Insert
    PhyB
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160603-plasmid-map-sequence-id-322052

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  16. Z-AIMP1-1

    Plasmid
    #166560
    Purpose
    scFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166560-plasmid-map-sequence-id-329268

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  17. G-BRD4-4

    Plasmid
    #166518
    Purpose
    scFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 44-168 of 1362, Bromodomain 1.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166518-plasmid-map-sequence-id-329057

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  18. pAc5.1B-lambdaN-HA-DmARC1_N

    Plasmid
    #146978
    Purpose
    Insect Expression of DmARC1
    Depositor
    Elisa Izaurralde
    Insert
    DmARC1 (Arc1 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    5 silent mutations and one non silent mutation G3…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146978-plasmid-map-sequence-id-389936

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  19. pT7-V5-SBP-C1-HsUpf1-del119-272-siRNAres_P

    Plasmid
    #147194
    Purpose
    Mammalian Expression of HsUpf1-del119-272-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsUpf1-del119-272-siRNAres (UPF1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    4 non silent mutations A69S, K282R, A442G and T1…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147194-plasmid-map-sequence-id-430439

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  20. pTH671-SUP35-H348Q

    Plasmid
    #29732
    Depositor
    Tobias von der Haar
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    H348Q mutant gene of SUP35 including genomic sequ…
    Promoter
    Available since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    29732-plasmid-map-sequence-id-13818

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Showing: 4881 - 4900 of 12887 results