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pAG1159 TOM20-ECFP-FIREmate

Plasmid

#196693

Purpose

Expresses TOM20-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

TOM20-ECFP-FIREmate

Tags

FIREmate

Expression

Mammalian

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-GMR-eya(shRNA)

Plasmid

#157991

Purpose

pBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.

Depositor

Greg Beitel

Article

Nyberg et al Fly (Austin). 2020 Oct 5. doi: 10.1080/1

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLJ57 (Pmec-17_GCaMP8f_SL2_mKate2_let-858_3'UTR)

Plasmid

#239865

Purpose

expression of GCaMP8f in C. elegans under mec-17-promoter control

Depositor

Monika Scholz

Article

Liu et al Genetics. 2024 Oct 7;228(2):iyae125. doi

Inserts

GCaMP8f
mKate2

Tags

6xHis

Expression

Worm

Mutation

codon optimized with introns for C. elegans

Promoter

mec-17

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Ptet1

Plasmid

#218367

Purpose

Empty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator site

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMIN-VS1

Plasmid

#197783

Purpose

Level 2 destination vector: pMIN-VS1 backbone

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCold CL7-Cas9

Plasmid

#124890

Purpose

obtaining full Cas9 RNP

Depositor

Yi Liu

Article

Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

Insert

Cas9

Expression

Bacterial

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT

Plasmid

#26106

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

6xHis, TEV cleavage site, and TrxA

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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mWasabi-C1

Plasmid

#54743

Purpose

Localization: C1 Cloning Vector, Excitation: 493, Emission: 509

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mWasabi

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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HH-gRNA-HDV-Shuttle-Vector

Plasmid

#169098

Purpose

Backbone plasmid to generate HH-gRNA-HDV segment

Depositor

Marianne Bronner

Article

Gandhi et al Development. 2021 Mar 9. pii: dev.193565

Insert

HH-gRNAbackbone-HDV

Expression

Mammalian

Promoter

NA

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

Plasmid

#175570

Purpose

Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Mutation

XbaI site added upstream of U6 promotor. G to C …

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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