We narrowed to 119,800 results for: %s

1. pML365_HSP90AA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227921

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   HSP90AA homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pML364_PSMB7-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227922

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   PSMB7 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pML374_CLTA-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227893

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   CLTA homology arms with mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pML307_SEC61B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227894

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   SEC61B homology arms with mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pML383_CCDC47-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227896

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   CCDC47 homology arms with mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pML384_NCLN-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227897

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   NCLN homology arms with mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227903

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   SEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.23-hs737

   Plasmid

   #222472

   Purpose

   Luciferase vector containing the hs737 enhancer sequence (reference sequence).

   Depositor

   Tychele Turner

   Article

   Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:

   Insert

   hs737 enhancer (reference sequence) (LOC110120928 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_SEPT9_i3 Gmut-msfGFP

   Plasmid

   #180326

   Purpose

   mammalian expression of human SEPT9_i3 Gmut fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN9_v3 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i3 W502A, H512D

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP

    Plasmid

    #180311

    Purpose

    bacterial expression of human SEPT2 NCmut fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    SEPT2 F20D, V27D

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_SEPT9_i3 NCmut-msfGFP

    Plasmid

    #180325

    Purpose

    mammalian expression of human SEPT9_i3 NCmut fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN9_v3 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i3 I263D, M270D

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. mTet1 flox donor

    Plasmid

    #117147

    Purpose

    Making Tet1 conditional knock out

    Depositor

    Jacob Hanna

    Article

    Zviran et al ssrn.3155731

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    Mouse Targeting

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. mTet3 flox donor

    Plasmid

    #117146

    Purpose

    Making Tet3 conditional knock out

    Depositor

    Jacob Hanna

    Article

    Zviran et al ssrn.3155731

    Insert

    Tet3 (Tet3 Mouse)
    

    Use

    Mouse Targeting

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET151-GyrA

    Plasmid

    #216229

    Purpose

    S. aureus USA300 GyrA Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET151-GyrB

    Plasmid

    #216233

    Purpose

    S. aureus USA300 GyrB Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-T3-st1CAS9-pA

    Plasmid

    #131542

    Purpose

    Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.

    Insert

    Codon optimized st1Cas9
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHsp70(K71E)-GFP

    Plasmid

    #228196

    Purpose

    expressed human Hsp70 (K71E mutation) with a GFP fluorescent protein tag

    Depositor

    Stephen Benkovic

    Article

    French et al Proc Natl Acad Sci U S A. 2013 Feb 12;11

    Insert

    HSP70(K71E) (HSPA1A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K71E

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNLF1-N_CCT5:1-541

    Plasmid

    #210978

    Purpose

    Mammalian expression of full-length human CCT5. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CCT5:M1-E541 (CCT5 Human)
    

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNLF1-C_CCT5:1-541

    Plasmid

    #210979

    Purpose

    Mammalian expression of full-length human CCT5. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CCT5:M1-E541 (CCT5 Human)
    

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET151-GyrA

    Plasmid

    #216229

    Purpose

    S. aureus USA300 GyrA Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge