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pBabe HcRed

Plasmid

#10677

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

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pAS1NB c Rosella I

Plasmid

#71245

Purpose

a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

Depositor

Mark Prescott

Article

Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

Insert

Rosella

Expression

Yeast

Promoter

PGK1

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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VCP(R155H)-EGFP

Plasmid

#23972

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

R155H

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pLJM1-Tmem192-mRFP-3xHA

Plasmid

#134631

Purpose

bait for affinity-isolation of lysosomes

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

3xHA, TEV, and mRFP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

Plasmid

#51082

Purpose

Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

Depositor

Jonathan Ting

Has Service

AAV1

Insert

GCaMP6s

Use

AAV

Tags

P2A-nls-dTomato

Expression

Mammalian

Promoter

hEF1a

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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Orai1 CFP

Plasmid

#19757

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 (ORAI1 Human)

Tags

CFP

Expression

Mammalian

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2_ubi:EGFP

Plasmid

#27323

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

ubiquitin:EGFP reporter

Use

Tol2 transposon

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP Myc

Plasmid

#59757

Purpose

encodes multiple copies of an epitope tag Myc

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _Myc

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK

Plasmid

#25033

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

Rck (DDX6 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV4-Tax WT

Plasmid

#23284

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

Tax

Expression

Mammalian

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only

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eGFP STAT1 Y701F

Plasmid

#12302

Depositor

Alan Perantoni

Article

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 Y701F (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Y701F

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pMV306hsp

Plasmid

#26155

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

hsp60 promoter

Use

Mycobacteria integrating vector

Available Since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pRosa26PAm1 (DM#272)

Plasmid

#15036

Depositor

Douglas Melton

Article

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Insert

pRosa26 modified targeting vector

Use

For making transgenic constructs

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-LC3 (Atg8)

Plasmid

#24919

Depositor

Toren Finkel

Article

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Insert

LC3 (MAP1LC3B Human)

Tags

Myc

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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Dominant negative Hsp90 beta

Plasmid

#22480

Depositor

William Sessa

Article

Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

Insert

Dominant negative Hsp90 (HSP90AB1 Human)

Tags

HA

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-human Dynamin 1aa

Plasmid

#22163

Depositor

Pietro De Camilli

Article

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

D744N

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV Myc T58A puro

Plasmid

#18773

Depositor

Scott Lowe

Article

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

myc T58A (MYC Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pCAG mito-RCaMP1h

Plasmid

#105013

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

RCaMP1h

Expression

Mammalian

Promoter

pCAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBPGAL4.2Uw-2

Plasmid

#26227

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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UbcH5c WT pET28a

Plasmid

#12643

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)

Expression

Bacterial

Available Since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

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