We narrowed to 32,141 results for: Eng

1. pTNSQCas

   Plasmid

   #219434

   Purpose

   CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

   Depositor

   Sheng Yang

   Article

   Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

   Insert

   TnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDonor-LoxP

   Plasmid

   #219436

   Purpose

   CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

   Depositor

   Sheng Yang

   Article

   Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

   Insert

   two transposon ends, LoxP-terminator-LoxP gene cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-hSyn-jGCaMP7b-XC

   Plasmid

   #182221

   Purpose

   Improved genetic encoded calcium sensor based on GCaMP7b.

   Depositor

   Xiaodong Liu

   Article

   Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

   Insert

   jGCaMP7b-XC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pc3xTC20-alphaTubulin

   Plasmid

   #36327

   Depositor

   Amy Palmer

   Article

   Van Engelenburg et al Chembiochem. 2010 Mar 1;11(4):489-93.

   Insert

   alpha tubulin 1b with optimized tetracysteine peptides for ReAsH labeling (TUBA1B Synthetic, Human)
   

   Use

   Tags

   3xTC20

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2a-copGFP(+HA)

   Plasmid

   #83806

   Purpose

   Express copGFP reporter for HDR-induced knock-in

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   copGFP
   

   Use

   Homologous recombination for gapdh loci

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-6p-1 Sesn2

   Plasmid

   #61873

   Purpose

   bacterial expression of a GST-Sesn2 (mouse)

   Depositor

   Ming Li

   Article

   Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

   Insert

   Sesn2 (Sesn2 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

7. Tol2-UAS-Dendra2

   Plasmid

   #97145

   Purpose

   For Gal4/UAS expression system

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   Dendra2
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS406 ATG9 stopGFP

   Plasmid

   #114669

   Purpose

   Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

   Insert

   ATG9
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Changed the three linker base pairs after the las…

   Promoter

   ATG9

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ-NF-L3-NF-L1-dMb2Cas12a

   Plasmid

   #177686

   Purpose

   Gateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editing

   Depositor

   Yiping Qi

   Article

   Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

   Insert

   2xFokI-dMb2Cas12a
   

   Use

   CRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PECAM-H2B-GFP

    Plasmid

    #14689

    Depositor

    Victoria Bautch

    Article

    Zeng et al Blood. 2007 Feb 15. 109(4):1345-52.

    Insert

    PECAM promoter/enhancer (Pecam1 Mouse)
    

    Use

    Tags

    H2B-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

11. L1-P941L

    Plasmid

    #15498

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-P941L (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Proline 941 changed to Leucine

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

12. Ef1a_NANOG_Ires_Puro

    Plasmid

    #18920

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    NANOG (NANOG Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

13. p3E-U6a-U6c

    Plasmid

    #107599

    Purpose

    An entry vector with U6a and U6c promoter driving guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Inserts

    U6a promoter
    U6c promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. L1-R184Q

    Plasmid

    #15501

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-R184Q (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Arginine 184 changed to Glutamine

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

15. EF.mHES1.Ubc.GFP

    Plasmid

    #17625

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    HES1 (Hes1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTrex-b-NLS-hSpCas9

    Plasmid

    #62543

    Purpose

    Expression of Cas9 in T. cruzi

    Depositor

    Rick Tarleton

    Article

    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

    Insert

    NLS-hSpCas9
    

    Use

    CRISPR; Trypanosoma cruzi expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Promoter

    Ribo-HX1

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSM_ser-2prom3p::myr-mCherry

    Plasmid

    #164223

    Purpose

    Expression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.

    Depositor

    Yan Zou

    Article

    Feng et al Cell Rep. 2020 Sep 8;32(10):108101. doi:

    Insert

    myr-mCherry
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    ser-2promo3

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. plenti-arRNA-BFP-backbone

    Plasmid

    #180221

    Purpose

    For the cloning of arRNA-expressing vectors containing BFP fluorescence marker in mammalian cells

    Depositor

    Wensheng Wei

    Article

    Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Oct4-ires-EGFP(lox neo)

    Plasmid

    #21547

    Depositor

    Rudolf Jaenisch

    Article

    Lengner et al Cell Stem Cell. 2007 Oct 11;1(4):403-15.

    Insert

    EGFP, Oct4
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L1-E309K

    Plasmid

    #15487

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-E309K (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Glutamic acid 309 changed to Lysine

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only