We narrowed to 24,999 results for: Nes

1. DMPK_HUMAN_D0

   Plasmid

   #79717

   Purpose

   This plasmid encodes the kinase domain of DMPK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   DMPK (DMPK Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. KC1G1_HUMAN_D0

   Plasmid

   #79691

   Purpose

   This plasmid encodes the kinase domain of KC1G1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KC1G1 (CSNK1G1 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. CSK22_HUMAN_D0

   Plasmid

   #79704

   Purpose

   This plasmid encodes the kinase domain of CSK22. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CSK22 (CSNK2A2 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pEN_TTmiRc2_3xflag_CHEK2

   Plasmid

   #136526

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   CHEK2 (CHEK2 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3x FLAG

   Expression

   Mutation

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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5. ML24

   Bacterial Strain

   #61915

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG asnA genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

6. ML25

   Bacterial Strain

   #61916

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG asnA asnB genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

7. ML26

   Bacterial Strain

   #61917

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

8. ML36

   Bacterial Strain

   #61919

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG metA genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

9. ML31

   Bacterial Strain

   #61918

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pNK3071

    Plasmid

    #219755

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNK497

    Plasmid

    #219754

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. C-Terminal Split Cas9 with GyrA intein

    Plasmid

    #58694

    Purpose

    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    

    Use

    AAV and CRISPR

    Tags

    GyrA Csplit Intein and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. N-Terminal Split Cas9 with GyrA intein

    Plasmid

    #58693

    Purpose

    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    

    Use

    AAV and CRISPR

    Tags

    3xFlag, GyrA Nsplit Intein, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-Cas9-2A-BFP

    Plasmid

    #164662

    Purpose

    Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Inserts

    Cas9
    BFP
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSSV_LTR

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-CBP FL

    Plasmid

    #179550

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-PHFF-sh-hBVRA

    Plasmid

    #100285

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    

    Use

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-GCN5 FL

    Plasmid

    #179552

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. LMPd Amt OXCT1

    Plasmid

    #209407

    Purpose

    Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

    Depositor

    Russell Jones

    Article

    Luda et al Immunity. 2023 Sep 12;56(9):2021-2035.e8

    Insert

    Oxct1 shRNA (Oxct1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ABL1_HUMAN_D0

    Plasmid

    #79727

    Purpose

    This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    ABL1 (ABL1 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. G protein alpha-s-YFP

    Plasmid

    #55781

    Purpose

    This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    alpha-s-EE YFP (Gnas Rat)
    

    Use

    Tags

    EYFP flanked by SGGGS on each side was inserted i…

    Expression

    Mammalian

    Mutation

    Met was substituted for Gln69 in EYFP (Clontech).…

    Promoter

    CMV

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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