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pcDNA3.1_SNORD60 probe

Plasmid

#73071

Purpose

antisense probe for detection of human SNORD60 (C/D box snoRNA U60)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD60 (SNORD60 Human)

Expression

Mammalian

Promoter

t7

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
hHSF4b K294R

Plasmid

#118357

Purpose

This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hHSF4b (HSF4 Human)

Expression

Mammalian

Mutation

K294R

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Gal4-HSF4b WT

Plasmid

#118358

Purpose

This plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hHSF4b (HSF4 Human)

Expression

Mammalian

Mutation

WT

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pJL15

Plasmid

#117430

Purpose

Expression of ARL2

Depositor

Richard Kahn

Article

Clark et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

ARL2 (ARL2 Human)

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pOU2

Plasmid

#41754

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc E. coli, Fly)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S C451S

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shLARP7

Plasmid

#113537

Purpose

Retroviral vector designed to knock down LARP7.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shLARP7 (LARP7 Human)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-MePCE_aa470-689

Plasmid

#113544

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 470 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa432-689

Plasmid

#113543

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 432 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa400-689

Plasmid

#113542

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 400 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa1-400

Plasmid

#113541

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 1 - 400.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-D27S/Y100F

Plasmid

#110827

Purpose

Bacterial expression of E.coli DHFR D27S/Y100F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

Insert

ecDHFR

Expression

Bacterial

Mutation

D27 mutated to S27, Y100 mutated to F100

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)

Plasmid

#107739

Purpose

mutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein(C1A)

Expression

Worm

Mutation

change the first cysteine of gp41-1 N-intein to a…

Promoter

C. elegans myo-2 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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p415 δCLD2α

Plasmid

#73874

Purpose

δCLD2α - His tagged (μHD deleted)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Arcn1/ δCLD2α (ARCN1 Bovine)

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG245-Ras-related

Plasmid

#99079

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Ras-related (also known as h.18.4a)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Ras-related (also known as h.18.4a) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMGF172

Plasmid

#96998

Purpose

ish1 mCherry insertion cassette with ura4+ gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + ura4(…

Promoter

N/A

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAM5081

Plasmid

#85107

Purpose

Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

YFP-KaiC

Tags

YFP

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pTYB3-human PUM1-HD MUT7-1 (E1083Q)-intein-CBD

Plasmid

#73303

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Mutation

E1083Q

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pTYB3-human PUM1-HD MUT7-2 (S1079N-E1083Q)-intein-CBD

Plasmid

#73299

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Mutation

S1079N-E1083Q

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pRFP shRNA-resistant TrkB F616A -S478A

Plasmid

#72718

Purpose

Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant and lacking TIAM1-binding site

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

TrkB

Tags

RFP

Expression

Mammalian

Mutation

shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 P50L

Plasmid

#60533

Purpose

Donor vector for human Pak5 P50L mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

P50L

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only