We narrowed to 45,377 results for: cha

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RcSxph Q787E

Plasmid

#194038

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Expression

Insect

Mutation

Q787E

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph K789A

Plasmid

#194039

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Expression

Insect

Mutation

K789A

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph D794A

Plasmid

#194040

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Expression

Insect

Mutation

D794A

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph D794E

Plasmid

#194041

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Expression

Insect

Mutation

D794E

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph D794N

Plasmid

#194042

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Expression

Insect

Mutation

D794N

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph Y795A

Plasmid

#194043

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Expression

Insect

Mutation

Y795A

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph Y558A/I782A

Plasmid

#194044

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Expression

Insect

Mutation

Y558A/I782A

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1-TwinStrep-His

Plasmid

#210816

Purpose

Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

TwinStrep, 10xHis

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1-StayGold

Plasmid

#210821

Purpose

Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

StayGold

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2H12ref

Plasmid

#221188

Purpose

cdGreen expressed in operon with mScarlet-I from an aTc-inducible promoter (Internal Lab ID: UJ11206)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

None (same as cdGreen) and Ptet

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR88

Plasmid

#203422

Purpose

empty BLINCAR plasmid for payload delivery, no payload, no genomic target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

CAR copy 1
P(TEF1)/coKanMX/T(ACT1) + P(TDH3)/coCBG/T(ADH2)
CAR copy 2

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR23

Plasmid

#202770

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coKanMX
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR10

Plasmid

#202710

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coShBle
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR9

Plasmid

#202645

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP

Plasmid

#207024

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

D384A Y387A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP

Plasmid

#207023

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

W371A I375A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-Y387A-GFP

Plasmid

#207022

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

Y387A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-D384A-GFP

Plasmid

#207021

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

D384A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-I375A-GFP

Plasmid

#207020

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

I375A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-W371A-GFP

Plasmid

#207019

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

W371A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMCSG7-10xHis-ecRNHI*

Plasmid

#219523

Purpose

bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

Depositor

Arthur Palmer

Article

Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

Insert

cysteine-free variant of the E. coli ribonuclease HI gene

Tags

N terminal 10xHis Tag

Expression

Bacterial

Mutation

Cysteine free variant

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-BFP-P2A-ppViperin

Plasmid

#217481

Purpose

Expression of Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Article

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

BFP-P2A-otPKRFL

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

Plasmid

#179717

Purpose

Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMS110

Plasmid

#215674

Purpose

Split hygromycinR landing pad insertion plasmid for ChrIII

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + synthetic guide site3 + 3'ΔHygR:unc-54 3' UTR + lox2272 + SEC + lox2272 + 3'HA

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

3' ∆HYGR is promoterless and encodes aa60-341

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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NFAST(low)-Halo-ER

Plasmid

#214419

Purpose

ER localization of low-affinity NFAST fused to Halo-ER

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 (96-421)

Plasmid

#213619

Purpose

Bacterial expression of SUMO-tagged rat syt1 (96-421)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 (96-421) (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 (96-421) I367C

Plasmid

#213620

Purpose

Bacterial expression of SUMO-tagged rat syt1 (96-421) I367C

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 (96-421) I367C (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

C277A; I367C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt7 (136-403)

Plasmid

#213621

Purpose

Bacterial expression of SUMO-tagged mouse syt7 (136-403)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 (136-403) (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 5W

Plasmid

#213623

Purpose

Bacterial expression of SUMO-tagged rat syt1 5W

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 5W (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

M173W, F231W, F234W, C277A, V304W, Y364W, I367C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 s7+

Plasmid

#213624

Purpose

Bacterial expression of SUMO-tagged rat syt1 s7+

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 s7+ (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

L171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt7 C2AB - 4A

Plasmid

#213625

Purpose

Bacterial expression of SUMO-tagged mouse syt7 C2AB - 4A

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 C2AB - 4A (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Mutation

F167A, F229A, I298A, L361A

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt7 C2A-2A 2B

Plasmid

#213626

Purpose

Bacterial expression of SUMO-tagged mouse syt7 C2A-2A 2B

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 C2A-2A 2B (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Mutation

F167A, F229A, R363H

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET SUMO-syt7 C2AB-2A

Plasmid

#213627

Purpose

Bacterial expression of SUMO-tagged mouse syt7 C2AB-2A

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 C2AB-2A (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Mutation

I298, L361A

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET SUMO-FL-syt1 5W

Plasmid

#213629

Purpose

Bacterial expression of SUMO-tagged rat FL-syt1 5W

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt1 5W (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

M173W, F231W, F234W, C277A, V304W, Y364W, I367C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-P1K-3VSV-Atg8-MET15

Plasmid

#207055

Purpose

Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Promoter

pATG8

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-Sik-YF

Plasmid

#210723

Purpose

Construct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.

Depositor

Angela Tyner

Article

Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

Insert

Protein tyrosine kinase 6 (Ptk6 Mouse)

Expression

Mammalian

Mutation

Tyrosine 447 changed to Phenylalanine

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-hXRCC1pd-HIS

Plasmid

#206036

Purpose

Bacterial expression of PAR-deficient hXRCC1pd with 6xHis tag, under the control of T7 promoter

Depositor

Primo Schaer

Article

Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

Insert

hXRCC1

Tags

HIS

Expression

Bacterial

Mutation

S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

Promoter

T7

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWR54

Plasmid

#203330

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

Plasmid

#209016

Purpose

Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Lentiviral

Tags

msfGFP

Expression

Mammalian

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSyt1C2AB(80 JuxtaK-421)msGFP

Plasmid

#209011

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis and msfGFP

Expression

Bacterial

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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