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Plasmid#231618PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS1 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS3
Plasmid#231617PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS3 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS2
Plasmid#231616PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS2 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
lvl0-I-2xcHS4
Plasmid#229160Purposelvl 0 insulatorDepositorInsert2xcHS4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
lvl0-I-inert
Plasmid#229159Purposelvl 0 insulatorDepositorInsertinert
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
NFfshb-t2a-GFP
Plasmid#194356Purposeover expression vector for killifish fshb tagged with GFPDepositorInsertfollicle stimulating hormone beta subunit
UseLentiviralTagst2a-GFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
SJZ6-HIS5
Plasmid#228259PurposeTemplate plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)DepositorInserthis5
UseTagsExpressionYeastMutationPromoterTEF1 S.c.Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)
Plasmid#225437PurposeTranscriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.DepositorInsertCBS-att-OCS-BeYDVRep/RepA-tNos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684)
Plasmid#225439PurposeModule for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.DepositorInsertHygR-MAR10-gemiGFP-MAR10
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-STK17A-V5-miniTurbo_IDG-K
Plasmid#214466PurposeGateway destination clone of STK17A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertSTK17A-V5-miniTurbo (STK17A Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
ROPY-4A
Plasmid#128005PurposeUnphosphorylatable mutant of Stathmin/OP18 FRET-sensorDepositorInsertROPY-4A
UseTagsExpressionMammalianMutationS16A, S25A, S38A, S63A in OP18PromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
cisRAJ31-cat
Plasmid#69933PurposeExpresses cisRAJ31-cat, a cis-repressed mRNA regulated by RAJ31.DepositorInsertPllacO1-cisRAJ31-cat
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlLacO1Available sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19_extendedTTE1564
Plasmid#60999PurposeT7-transcription template for Tte mRNA used in SiM-KARTSDepositorInsertTTE1564 transcript
UseUnspecifiedTagsExpressionMutationPromoterT7Available sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMPO 51
Plasmid#68358PurposeExpression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2). ApR, expression vector with rrnBT1T2-Pm-MCSII, ColE1 replication originDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 to gap
Plasmid#49158PurposeTemplate for generating gene 60 mRNA, sequence 5' of coding gap onlyDepositorInsertBacteriophage T4 gene 60
UseTagsExpressionMutationCoding gap and downstream sequence have been del…PromoterT7Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRAJ12f
Plasmid#39248DepositorInsertRAJ12 system (no terminator for the transRNA)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1+pLtetO-1Available sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRAJ22
Plasmid#39251DepositorInsertRAJ22 system
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1+pLtetO-1Available sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRAJ22m
Plasmid#39252DepositorInsertRAJ22 system (no transRNA)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1Available sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRAJ23
Plasmid#39253DepositorInsertRAJ23 system
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1+pLtetO-1Available sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRAJ23m
Plasmid#39254DepositorInsertRAJ23 system (no transRNA)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1Available sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only