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pAAV-FLEX-hKCNQ3-P2A-EGFP

Plasmid

#209195

Purpose

Cre-dependent expression of Kcnq3 and GFP from the same transcript

Depositor

Larry Zweifel

Article

Gamal El-Din et al Proc Natl Acad Sci U S A. 2021 Nov 9;118

Insert

Kcnq3 (KCNQ3 Human)

Use

AAV

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-γ-tubulin

Plasmid

#136799

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-TUBG (TUBG1 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pQE80L-SpyCatcher-ELP-GFP

Plasmid

#69835

Purpose

Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

Insert

SpyCatcher-ELP-GFP

Tags

6x His Tag, GFP, and TEV Tag

Expression

Bacterial

Promoter

T5 promoter/lac operator element

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 TFAM-mScarlet

Plasmid

#129573

Purpose

Expression of mScarlet fused TFAM

Depositor

Stephen Tait

Article

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

TFAM (TFAM Human)

Expression

Mammalian

Mutation

None

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

Plasmid

#183411

Purpose

piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

Tet-on 3G

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1A

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-OSBP

Plasmid

#232871

Purpose

Expresses OSBP in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

mCherry-OSBP (OSBP Human)

Tags

mCherry

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKalpha2 wt

Plasmid

#31654

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-cMyc

Plasmid

#46970

Purpose

lentiviral expression of c-Myc

Depositor

Jialiang Wang

Article

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCDH-Flag-c-Myc

Plasmid

#102626

Purpose

Expresses c-Myc in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

MYC proto-oncogene, bHLH transcription factor (MYC Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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hCathepsin B

Plasmid

#11249

Purpose

Mammalian expression of human cathepsin B

Depositor

Hyeryun Choe

Article

Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

Insert

cathepsin B (CTSB Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pIVM_26

Plasmid

#204501

Purpose

Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

Depositor

Alessio Ciulli

Article

Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

Insert

EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)

Expression

Bacterial

Mutation

0

Promoter

AmpR

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHSP90(G97D)-mOFP

Plasmid

#228195

Purpose

expressed human Hsp90 (G97D mutation) with a mOFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

French et al Proc Natl Acad Sci U S A. 2013 Feb 12;11

Insert

HSP90AA1(G97D) (HSP90AA1 Human)

Tags

mOFP

Expression

Mammalian

Mutation

G97D mutation

Promoter

CMV

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin

Plasmid

#60613

Purpose

Encodes YFP and NLS tagged beta-actin

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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Flag Leucyl tRNA synthetase pRK5

Plasmid

#46341

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

leucyl tRNA synthetase (LARS1 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pHAGE-N-FLAG-HA-TBK1

Plasmid

#131791

Purpose

Lentiviral vector expressing WT TBK1

Depositor

Alicia Pickrell

Article

Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

Insert

TBK1 WT (TBK1 Human)

Use

Lentiviral

Tags

N-FLAG-HA

Expression

Mammalian

Mutation

WT full length

Promoter

CMV

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 TFAM-mClover

Plasmid

#129574

Purpose

Expression of mClover fused TFAM

Depositor

Stephen Tait

Article

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

TFAM (TFAM Human)

Expression

Mammalian

Mutation

None

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hTRF2

Plasmid

#103804

Purpose

BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hTRF2 (CIB1, TERF2 Mustard Weed, Human)

Expression

Mammalian

Mutation

hTRF2: deletion of amino acids 1-42 and 476 compa…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-IRF4-FLAG-IRES-GFP

Plasmid

#107389

Purpose

Lentiviral expression of IRF4-FLAG

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IRF4 (IRF4 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDragon-Ctgf

Plasmid

#155015

Purpose

For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Insert

Ccn2 (Ccn2 Mouse)

Use

Mouse Targeting

Mutation

M24T (please see depositor comments)

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/DEST/hTfR-HA

Plasmid

#69610

Purpose

human transferrin receptor with an HA tag fused at the C-terminus

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

human transferrin receptor (TFRC Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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