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pMS21

Plasmid

#229476

Purpose

Expresses RssB with E135A, E136A, & E137A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Tags

10xHis

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF18

Plasmid

#229456

Purpose

Expresses IraD (aa17-130) with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (aa 17-130 only)

Tags

6xHis

Expression

Bacterial

Mutation

aa 17-130 only

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF-His_TEV_MsbA

Plasmid

#191476

Purpose

Expresses MsbA with a TEV protease cleavable N terminal His Tag

Depositor

Arthur Laganowsky

Article

Lyu et al Nat Commun. 2022 Nov 26;13(1):7291. doi:

Insert

MsbA

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-EcYRS-EGFP*

Plasmid

#191118

Purpose

A plasmid for incorporation of O-Me-Tyr into an EGFP reporter for zebrafish

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein

Tags

His tag

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_LmFPPS

Plasmid

#186322

Purpose

pET-28a vector (Novagen) (encoding an N-terminal His tag), to transform Escherichia coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Aripirala et al Acta Crystallogr D Biol Crystallogr. 201

Insert

FPPS

Tags

His Tag

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-Tir

Plasmid

#221326

Purpose

Dox-inducible expression of Tir in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

Tir

Use

Retroviral

Tags

HN-FLAG

Promoter

TRE3GV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speG_genomic

Plasmid

#220473

Purpose

heterologous expression under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.1

Plasmid

#208306

Purpose

Expresses ABE-RA4.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.1-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.3

Plasmid

#208308

Purpose

Expresses ABE-RA4.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.3-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.4

Plasmid

#208309

Purpose

Expresses ABE-RA4.4in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.4-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.5

Plasmid

#208310

Purpose

Expresses ABE-RA4.5 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.5-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.6

Plasmid

#208311

Purpose

Expresses ABE-RA4.6 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.6-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.0

Plasmid

#208312

Purpose

Expresses ABE-RA5.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.0-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.1

Plasmid

#208313

Purpose

Expresses ABE-RA5.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.1-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.3

Plasmid

#208314

Purpose

Expresses ABE-RA5.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.3-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.4

Plasmid

#208315

Purpose

Expresses ABE-RA5.4 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.4-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, R47K, P48A, R51L, I76Y, V82S, A106V, D108G,…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.6

Plasmid

#208317

Purpose

Expresses ABE-RA5.6 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.6-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-HO1(51-231)

Plasmid

#203486

Purpose

For recombinant protein production in Escherichia coli. His-tagged Arabidopsis thaliana HEME OXYGENASE 1 (HO1) without the plastid transit peptide under the control of the T7 promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Arabidopsis thaliana HEME OXYGENASE1 (51 aa – 231 aa)

Expression

Bacterial and Plant

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP24_T24_pRham-ilvA-relE_RBS3

Plasmid

#201532

Purpose

Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE_RBS3

Use

Synthetic Biology

Promoter

PrhaBAD (rhamnose)

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2572

Plasmid

#195683

Purpose

Expression/purification of 6xHis-TEV-MlaC(V171R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(V171R)

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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