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p3E-CMVmin:GFPmem no-pA

Plasmid

#80824

Purpose

CMVmin-promoted GFPmem without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

CMV-GFPCAAX nopA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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POT4-TEF2p-ycrtI-ADH1t

Plasmid

#65356

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pDESTFLAGHApuro3-Ago Chi-b

Plasmid

#14085

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-b

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-b

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago Chi-c

Plasmid

#14086

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-c

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-c

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
mg172

Plasmid

#34652

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A1;B1;C0) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pGLx2-set1deltaRRM

Plasmid

#53257

Purpose

GAL driven LexA-tagged SET1-deltaRRM (RRM domain deleted)

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Tags

LexA

Expression

Yeast

Mutation

RRM domain deleted (aa 230-355)

Promoter

GAL1/10

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLSLZ.D

Plasmid

#51496

Purpose

pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

SV40 NLS

Promoter

virion-sense LIR and 2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pPK700

Plasmid

#38146

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

let-60p::ptc-3::gfp (ptc-3 Nematode)

Tags

EGFP

Expression

Worm

Promoter

let-60

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pET3a-AqAdk_V145C

Plasmid

#18091

Depositor

Dorothee Kern

Article

Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

Insert

adenylate kinase (kad A. aeolicus)

Expression

Bacterial

Mutation

Val 145 changed to Cys

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
XE169 mouse Wnt-1-myc-pGEM4

Plasmid

#16855

Depositor

Randall Moon

Article

McMahon et al Cell. 1989 Sep 22;58(6):1075-84. doi: 10

Insert

Wnt-1-myc (Wnt1 Mouse)

Use

Xenopus expression

Tags

Myc

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago Chi-d

Plasmid

#14087

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-d

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-d

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pLCHKO-CRASP

Plasmid

#231928

Purpose

Empty backbone for CRASP-Seq experiments

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Type

Empty backbone

Use

Lentiviral

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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p304N

Plasmid

#246317

Purpose

Plant CRISPR editing with the experimentally validated Medicago truncatula MtU6.6 promoter (352 bp)

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pJP_Bla01

Plasmid

#230982

Purpose

Modified version of the pBLADE_ONLY_C (#168050). It expresses VVD-AraC, but the f1 ori was deleted and the chloramphenicol resistance gene was replaced by gentamicin resistance.

Depositor

Yolanda Schaerli

Article

Hollo et al Mol Syst Biol. 2025 Sep 29. doi: 10.1038

Insert

Gentamicin resistance gene

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pJP_1node

Plasmid

#230984

Purpose

pBAD promoter controls the expression of sfGFP. In the presence of VVD-AraC, blue light and L-arabinose can be used to activate mCitrine expression.

Depositor

Yolanda Schaerli

Article

Hollo et al Mol Syst Biol. 2025 Sep 29. doi: 10.1038

Insert

mCitrine

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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Ntom-Venus-ssrA

Plasmid

#223688

Purpose

PhoBIT1 component; Venus-tagged ssrA tag fused with Ntom for mitochondrial tethering

Depositor

Yubin Zhou

Article

Lee et al Nat Commun. 2025 Jul 28;16(1):6940. doi:

Insert

ssrA peptide

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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mCh-sspB(LOV2)

Plasmid

#223689

Purpose

PhoBIT1 component; mCherry-tagged sspB with LOV2 insertion

Depositor

Yubin Zhou

Article

Lee et al Nat Commun. 2025 Jul 28;16(1):6940. doi:

Insert

sspB(N)-LOV2-sspB(C)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-KO-delVP1-VP1-588TAG

Plasmid

#240123

Purpose

Express AAV2-KO (R585A,R588A) capsid with ncAA mutant at site 588 of VP1

Depositor

Abhishek Chatterjee

Article

Pham et al bioRxiv 2024.07.20.604406

Inserts

RC2-DelVP1-R585,588A
VP1-588-TAG

Use

AAV

Expression

Mammalian

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5425

Plasmid

#229032

Purpose

Expression vector for 10xHis-phi29 DNA polymerase (catalytically inactive mutant D249E): PT7-lacO-g10leader-p2 dead-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP (D249E, catalytically inactive)

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-pCAG-iCRE-2A-H2BGFP-WPRE

Plasmid

#234893

Purpose

CAG-driven Cre recombinase coupled with H2B-GFP for nuclear fluorescent labelling

Depositor

Silvia Arber

Article

Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

Insert

iCRE-2A-H2BGFP

Use

AAV

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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