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pEGFPC1-hVAP-A

Plasmid

#104447

Purpose

Expression of human VAP-A fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTCB-Sig7R124AFcholeHA

Plasmid

#237368

Purpose

pTwist CMV BetaGlobin encoding Siglec-7-Fc R124A mutant with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

Siglec-7-Fc (R124A mutant) (SIGLEC7 Human)

Use

Affinity Reagent/ Antibody

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-B

Plasmid

#104448

Purpose

Expression of human VAP-B fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Tags

EGFP and HA

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTCB-Gal9NFcLALAPGhole6xHis

Plasmid

#237381

Purpose

pTwist CMV BetaGlobin encoding Galectin-9 N terminal carbohydrate binding domain-Fc fusion with LALA P329G hole Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol 2025

Insert

Galectin-9-silentFc (N-terminal carbohydrate binding domain only) (LGALS9 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pUN1214 - pL0_pNMT (pro + 5U)

Plasmid

#203893

Purpose

NMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

NMT promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI and/or BsaI cut sites for MoClo …

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-A KD/MD

Plasmid

#104449

Purpose

Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Mutation

changed K94 to D and M96 to D

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-B KD/MD

Plasmid

#104450

Purpose

Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

changed K87 to D and M89 to D

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001144864)

Plasmid

#77563

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001148670)

Plasmid

#77564

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001146365)

Plasmid

#77565

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001144918)

Plasmid

#77566

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KHDRBS3_KH_pGEX

Plasmid

#135133

Purpose

Recombinant expression of KHDRBS3 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

KHDRBS3 (KHDRBS3 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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pMT316

Plasmid

#8608

Depositor

Robert Hartley

Article

Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

Insert

barstar

Expression

Bacterial

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pMT413

Plasmid

#8606

Depositor

Robert Hartley

Article

Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

Insert

barnase, barstar

Tags

phoA signal sequence to barnase

Expression

Bacterial

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pMT416

Plasmid

#8607

Depositor

Robert Hartley

Article

Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

Insert

barnase, barstar

Tags

phoA signal sequence to barnase

Expression

Bacterial

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pSW_1

Plasmid

#71279

Purpose

Gateway destination vector

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Type

Empty backbone

Use

Gateway vector

Tags

GAL4 DBD

Promoter

CMV

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_240_pAGW_V5

Plasmid

#71188

Purpose

Gateway destination vector

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Type

Empty backbone

Use

Gateway

Tags

Gal4 DBD and V5

Promoter

actin 5C

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pStA1BZ

Plasmid

#114164

Purpose

Start-Stop Assembly Level 1BZ plasmid

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA1BC

Plasmid

#114165

Purpose

Start-Stop Assembly Level 1BC plasmid

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA1CZ

Plasmid

#114166

Purpose

Start-Stop Assembly Level 1CZ plasmid

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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