Addgene

We narrowed to 1,461 results for: asl;

Showing: 481 - 500 of 1461 results

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Human USP30 (64-502, construct 2, MG-26-18)

Plasmid

#110744

Purpose

Bacterial expression of human USP30 (construct 2)

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Use

Tags

N-His6-3C-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPH1-mEGFP

Plasmid

#105270

Purpose

Plasmid for pop-in/pop-out replacement of Vph1 with Vph1-mEGFP.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vph1 portion (VPH1 Budding Yeast)

Use

Tags

mEGFP

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCSC-SP-PW-NepX (aka: pBOB-NEPX)

Plasmid

#12340

Depositor

Inder Verma

Article

Marr et al J Mol Neurosci. 2004 . 22(1-2):5-11.

Insert

NEP-X (MME Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E585V (inactive mutant)

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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poRFP-oGFP

Plasmid

#154130

Purpose

Expresses orthogonal gfp and orthogonal rfp genes in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Inserts

orthogonal gfp
orthogonal rfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS10-msGFPx3

Plasmid

#105274

Purpose

Plasmid for pop-in/pop-out replacement of Vps10 with Vps10-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps10 portion (PEP1 Budding Yeast)

Use

Tags

msGFPx3

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC7-mCherry2Bx6

Plasmid

#105267

Purpose

Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Sec7 portion (SEC7 Budding Yeast)

Use

Tags

mCherry2Bx6

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Human USP30 (1-517, C77A, MG-38-16)

Plasmid

#110749

Purpose

Transient transfection of human USP30 C77A

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Use

Tags

Expression

Mammalian

Mutation

C77A

Promoter

CMV

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac204-T|C-iGFP-PH(OSH1)

Plasmid

#105280

Purpose

Overexpresses iGFP-PH(Osh1) under the TPI1 promoter. Integrating at TRP1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

PH domain of Osh1 (SWH1 Budding Yeast)

Use

Tags

iGFP

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-mCherry2Bx6

Plasmid

#105273

Purpose

Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-mCherry2Bx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps8 portion (VPS8 Budding Yeast)

Use

Tags

mCherry2Bx6

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC7-msCFPx6

Plasmid

#105268

Purpose

Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-msCFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Sec7 portion (SEC7 Budding Yeast)

Use

Tags

msCFPx6

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-GGA2-msGFPx3

Plasmid

#105258

Purpose

Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Gga2 portion (GGA2 Budding Yeast)

Use

Tags

msGFPx3

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL2-iGFPx6

Plasmid

#105251

Purpose

Plasmid for pop-in/pop-out replacement of Apl2 with Apl2-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

APL2 portion (APL2 Budding Yeast)

Use

Tags

iGFPx6

Expression

Yeast

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL5-mCherry2Bx6

Plasmid

#105253

Purpose

Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-mCherry2Bx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl5 (APL5 Budding Yeast)

Use

Tags

mCherry2Bx6

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-PIK1

Plasmid

#105260

Purpose

Plasmid for pop-in/pop-out replacement of Pik1 with iGFP-Pik1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Pik1 (PIK1 Budding Yeast)

Use

Tags

iGFP

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-iYFPx6

Plasmid

#105272

Purpose

Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iYFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps8 portion (VPS8 Budding Yeast)

Use

Tags

iYFPx6

Expression

Yeast

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL6-msGFPx3

Plasmid

#105254

Purpose

Plasmid for pop-in/pop-out replacement of Apl6 with Apl6-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl6 portion (APL6 Budding Yeast)

Use

Tags

msGFPx3

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-iGFPx6

Plasmid

#105271

Purpose

Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps8 portion (VPS8 Budding Yeast)

Use

Tags

iGFPx6

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-CHC1-msGFPx3

Plasmid

#105256

Purpose

Plasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Chc1 portion (CHC1 Budding Yeast)

Use

Tags

msGFPx3

Expression

Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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LARS_pNIC-Bio3

Plasmid

#153052

Purpose

Expression construct for producing Avi tagged Leucyl-tRNA synthetase

Depositor

Susanne Gräslund

Insert

LARS (LARS1 Human)

Use

Tags

Avi and His6-TEV

Expression

Bacterial

Mutation

Not full length protein

Promoter

T7

Available since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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poRBS

Plasmid

#154125

Purpose

Expresses orthogonal rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Insert

engineered bacterial rRNAs operon under control of pL promotor

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Showing: 481 - 500 of 1461 results

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