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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 846 results for: cas9 expression construct

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Showing: 481 - 500 of 846 results

  2. MTK234_055

    Plasmid
    #123920
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123920-plasmid-map-sequence-id-250070

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  10. MTK234_055

    Plasmid
    #123920
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123920-plasmid-map-sequence-id-250070

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  16. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Expression
    Mammalian
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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  17. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid
    #124537
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41 C55G, d11-41 C55G
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124537-plasmid-map-sequence-id-242309

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  18. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid
    #124542
    Purpose
    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41 C55G, d11-41 C55G
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124542-plasmid-map-sequence-id-242329

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  19. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid
    #124543
    Purpose
    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41 C55G, d11-41
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124543-plasmid-map-sequence-id-242336

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  20. pHDR-eGFP (pXK-1647)

    Plasmid
    #208824
    Purpose
    The HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDR
    Depositor
    Kun Xu
    Article
    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208824-plasmid-map-sequence-id-417244

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Showing: 481 - 500 of 846 results