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Plasmid#98297PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS1017-Tier1-OTetO7-PCMVmin-SEAP
Plasmid#169586PurposeTier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).DepositorInserttetracycline-controlled SEAP expression
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVminAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Actinin-sstFRET-GR
Plasmid#83416PurposeFRET-based actinin tension sensorDepositorInsertActinin-sstFRET-GR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHB-3
Plasmid#140956PurposeFor PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.DepositorInsertphaCAB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK01_PalkS_alkS
Plasmid#166502PurposeExpression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertalkS
UseSynthetic BiologyTagsExpressionBacterialMutationL401GPromoterPalkSAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB_PalkB_sfgfp_KanR
Plasmid#166503PurposeExpression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPalkBAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
ARK2aa
Plasmid#81013PurposeE.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-aphA
and PMD
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK792
Plasmid#8830DepositorInsertTEV protease, S219P mutant
UseTagsHis, MBP (cleaved), and polyarginineExpressionBacterialMutationS219P mutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRC3EGFP
Plasmid#193790PurposeExpresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterPR promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT-MBI (JBEI-3085)
Plasmid#87950Purposethe overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).DepositorInsertMevT-MBI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-pVhb-tdTER-fdh
Plasmid#215649PurposepBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoterDepositorInsertstrans-enoyl-CoA reductase
formate dehydrogenase
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVH233-Tier1-PmPGK1-eYFP
Plasmid#169522PurposeTier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).DepositorInsertPPGK-driven enhanced yellow fluorescent protein
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH231-Tier1-PmPGK1-mTagBFP2
Plasmid#169520PurposeTier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).DepositorInsertPPGK-driven monomeric Tag blue fluorescent protein 2
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCW012
Plasmid#83527PurposeCloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminatorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVH607-Tier1-PCREm4-Nluc
Plasmid#169594PurposeTier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).DepositorInsertcAMP response element-controlled NanoLuc Luciferase expression cassette
UseTagsExpressionMammalianMutationPromoterPCREm4Available sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTRCk-pVhb-maACR
Plasmid#215651PurposepBBR1 Origin, KanR, with maACR gene under Vhb promoterDepositorInsertacyl-CoA reductase
UseTagsMaltose binding protein and His tagExpressionBacterialMutationPromoterVhbAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-T7Max-UTR1-deGFP-8xHis-T500
Plasmid#178422PurposeExpression of deGFP via a T7 Promoter in TXTLDepositorInsertdeGFP
UseSynthetic BiologyTags8xHis TagExpressionBacterialMutationPromoterT7MaxAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30b_T7Sav
Plasmid#138588PurposeT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus
UseSynthetic BiologyTagsT7-tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only