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pJW1659

Plasmid

#154314

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::AID*::3xFLAG for NT slot

Use

CRISPR

Expression

Worm

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1816

Plasmid

#154320

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1836

Plasmid

#154337

Purpose

Promoter reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMJA103

Plasmid

#128519

Purpose

Expresses human amyloid Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme D67H (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA104

Plasmid

#128520

Purpose

Expresses amyloid human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme I56T (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1820

Plasmid

#154321

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC (LoxP)^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1806

Plasmid

#154319

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::3xMyc for NT slot

Use

CRISPR

Expression

Worm

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1805

Plasmid

#154318

Purpose

CT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::3xMyc for CT slot

Use

CRISPR

Expression

Worm

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1822

Plasmid

#154322

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP

Use

CRISPR

Expression

Worm

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1827

Plasmid

#154323

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I

Use

CRISPR

Expression

Worm

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1841

Plasmid

#154338

Purpose

Promoter reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMJA105

Plasmid

#128521

Purpose

Expresses amyloid human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme T70N (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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dual-sgRNA CRISPRi library for PARP inhibitor response

Pooled Library

#238134

Purpose

This library can be used in genetic interaction experiments for mapping genetic interaction network in DNA repair and PARP inhibitor response.

Depositor

Brittany Adamson

Article

Simpson et al bioRxiv [Preprint]. 2023 Aug 20:2023.08.

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only
pJW1839

Plasmid

#154325

Purpose

sgRNA Destination Vector for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1582

Plasmid

#154312

Purpose

mNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mNeonGreen^SEC^AID*::3xFlag

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW2088

Plasmid

#154336

Purpose

Crispr repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1846

Plasmid

#154326

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP^SEC^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

F+E mutations in sgRNA

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1947

Plasmid

#154339

Purpose

Enhancer reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

pes-10delta::SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1886

Plasmid

#154333

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I^SEC (Lox511I)^TEV::degron::3xFLAG

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1788

Plasmid

#154317

Purpose

CT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::TEV::BioTag::AID*::3xFLAG donor for CT slot in the SapTrap cloning system

Use

CRISPR

Expression

Worm

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge