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pAtCas9-D10A_3

Plasmid

#91161

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A1410

Plasmid

#91035

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

ZmUbi

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTC440

Plasmid

#91225

Purpose

protoplast vector expressing gRNA38 targeting barley MLO (control without TREX2 expression)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting barley MLO

Use

CRISPR

Expression

Plant

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTC441

Plasmid

#91218

Purpose

protoplast vector for targeted deletion of MLO gene in barley (PvUbi1 promoter driving the gRNA array)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting MLO gene in barley

Use

CRISPR

Expression

Plant

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTC436

Plasmid

#91222

Purpose

protoplast vector expressing gRNA38 targeting barley MLO (with TREX2 expression)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting barley MLO

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTC437

Plasmid

#91219

Purpose

protoplast vector for targeted deletion of MLO gene in barley (CmYLCV promoter driving the gRNA array)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting MLO gene in barley

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE77

Plasmid

#80579

Purpose

recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
p2x35S:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE78

Plasmid

#80580

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tmos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE79

Plasmid

#80581

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCCL-NoPromoter-FLuc-CMV-RLuc-dsRed2

Plasmid

#215329

Purpose

Lentiviral mammalian vector with firefly luciferase gene with no upstream promoter sequence; renilla luciferase and dsRed2 reporter under CMV promoter.

Depositor

Rob Hynds

Article

Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

Insert

No Promoter

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pVTCL1

Plasmid

#234919

Purpose

All-in-one subcellar localization vector. Construct containing EGFP tag and cell nucleus-targeted mCherry for co-localization study in plants.

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVSr-GhOMT1

Plasmid

#234932

Purpose

This virus-induced gene silencing (VIGS) reporter vector is used to evaluate the silencing of GhOMT1 gene

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

Partial coding region sequence of GhOMT1

Expression

Plant

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pVO-NanoLUC

Plasmid

#234900

Purpose

This all-in-one vector is used to overexpress the NanoLUC gene via virus-mediated overexpression (VOX)

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

NanoLUC

Expression

Plant

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pVTCL2

Plasmid

#234920

Purpose

All-in-one subcellar localization vector. Construct containing EGFP tag and plasma membrane-targeted mCherry for co-localization study in plants.

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pVTCL1-AtbZIP63

Plasmid

#234923

Purpose

This all-in-one subcellar localization vector is used to study the nuclear localization of AtbZIP63

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

AtbZIP63

Expression

Plant

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pVTCL4

Plasmid

#234922

Purpose

All-in-one subcellar localization vector. Construct containing EGFP tag and Golgi-targeted mCherry for co-localization study in plants.

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pVTCL3

Plasmid

#234921

Purpose

All-in-one subcellar localization vector. Construct containing EGFP tag and cell endoplasmic reticulum-targeted mCherry for co-localization study in plants.

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pVTHV3-(AtbZIP63-nV+cV)

Plasmid

#234925

Purpose

This all-in-one bimolecular fluorescence complementation (BiFC) vector is used to study the self-interation of AtbZIP63 proteins

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

AtbZIP63-nV+cV

Expression

Plant

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pVTHV3-(nV+AtbZIP63-cV)

Plasmid

#234926

Purpose

This all-in-one bimolecular fluorescence complementation (BiFC) vector is used to study the self-interation of AtbZIP63 proteins

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

nV+AtbZIP63-cV

Expression

Plant

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pVSr-GhPDS

Plasmid

#234931

Purpose

This virus-induced gene silencing (VIGS) reporter vector is used to evaluate the silencing of GhPDS gene

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

Partial coding region sequence of GhPDS

Expression

Plant

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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