We narrowed to 12,887 results for: sequence

1. pTH672-SUP35-Y351C

   Plasmid

   #29733

   Depositor

   Tobias von der Haar

   Insert

   SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Y351C mutant gene of SUP35 including genomic sequ…

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP14801 - pAAV-AiE0390m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4801)

   Plasmid

   #208163

   Purpose

   AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Insert

   iCreR297T
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AiP13999 - pAAV-AiE0387m-minBG-iCre(R297T)-BGHpA (Alias: CN3999)

   Plasmid

   #208166

   Purpose

   AiE0387m is an enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Insert

   iCreR297T
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-MS2-HA-HsRRP6_Z

   Plasmid

   #148131

   Purpose

   Mammalian Expression of HsRRP6

   Depositor

   Elisa Izaurralde

   Insert

   HsRRP6 (EXOSC10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2 silent mutations compared to the sequence of is…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-CRISPR-Puro

   Plasmid

   #208646

   Purpose

   pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 in tandem

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGFP Cortactin

   Plasmid

   #50728

   Purpose

   mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Insert

   Cortactin (CTTN Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

   Plasmid

   #118156

   Purpose

   Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-puro U6 sgRNA BfuAI large stuffer

   Plasmid

   #52628

   Purpose

   lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency

   Depositor

   Scot Wolfe

   Insert

   Kanamycin cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO-Tet-ON-shCtrl (neo)

   Plasmid

   #192076

   Purpose

   Lentivirus for Dox-inducible expression of a non-targeting control shRNA

   Depositor

   Roland Friedel

   Insert

   non-targeting randomized sequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   H1 / Tet-responsive element

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-mNG-IRES-mScarlet-dMCP

    Plasmid

    #231089

    Purpose

    Bicistronic construct encoding mNG in combination with mScarlet-dMCP via an EMCV-IRES sequence.

    Depositor

    John Ngo

    Insert

    mNG-IRES-mScarlet-dMCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti spCas9 T2A iRFP670 P2A puro

    Plasmid

    #122182

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9 (NEWENTRY )
    iRFP670
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti spCas9 T2A mNeonGreen P2A puro

    Plasmid

    #122183

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9 (NEWENTRY )
    mNeonGreen
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-mNG-IRES-mScarlet-dPCP

    Plasmid

    #231090

    Purpose

    Bicistronic construct encoding mNG in combination with mScarlet-dPCP via an EMCV-IRES sequence.

    Depositor

    John Ngo

    Insert

    mNG-IRES-mScarlet-dPCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti spCas9 T2A TagRFP-T P2A puro

    Plasmid

    #122200

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9
    TagRFP-T
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLIK sh human Rb 1534 hyg

    Plasmid

    #31500

    Depositor

    Julien Sage

    Insert

    RB shRNA (RB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    The target sequence for shRB 1534 is GAACGATTATCC…

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS403-PGAL1-hpSC_URA3

    Plasmid

    #22317

    Depositor

    David Bartel

    Article

    Drinnenberg et al Science. 2009 Sep 10. ():.

    Insert

    URA hairpin
    

    Use

    Integrating plasmid

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. G-SMARCA4-2

    Plasmid

    #166519

    Purpose

    scFv of a human scaffold targeting SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a4 isoform B. Antigen coverage aa 1451-1580 of 1647, Bromodomain.

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1B-EGFP-DmAtx2_AE

    Plasmid

    #148548

    Purpose

    Insect Expression of DmAtx2

    Depositor

    Elisa Izaurralde

    Insert

    DmAtx2 (Atx2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Three silent mutations and two deletions compared…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AICSDP-77:TERF2-mEGFP

    Plasmid

    #168798

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human TERF2.

    Depositor

    Allen Institute for Cell Science

    Insert

    TERF2 (TERF2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    April 14, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. AiP13318 - pAAV-AiE0390m_3xC2-minBG-SYFP2-4x2C-WPRE3-BGHpA (Alias: CN3318)

    Plasmid

    #208167

    Purpose

    AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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