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NAX_CAGGS_CAS9_m4_VPR_EYFP

Plasmid

#167882

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_EBFP2

Plasmid

#167873

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP1F5

Plasmid

#165064

Purpose

Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator

Depositor

Claudia Vickers

Publication

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_OctaHis-GFP11

Plasmid

#153509

Purpose

OctaHis-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

OctaHis-GFP11

Use

Synthetic Biology

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB8382

Plasmid

#126916

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

Depositor

Irina Borodina

Publication

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8818

Plasmid

#126909

Purpose

Template for PCR amplification of HMG1from Yarrowia lipolytica

Depositor

Irina Borodina

Publication

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

YlHMG1

Use

Synthetic Biology

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgCh2-2

Plasmid

#125775

Purpose

constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgCh2-2

Use

CRISPR

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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T7-AglK-His

Plasmid

#89714

Purpose

Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

Depositor

Barbara Imperiali

Publication

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

AglK

Tags

His6 and T7

Expression

Bacterial

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSY091

Plasmid

#110068

Purpose

Bacterial expression of L4

Depositor

Jay Keasling

Publication

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L4

Mutation

AT-swapped mutant

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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glgC-

Plasmid

#101842

Purpose

deletes glgC locus

Depositor

Erin O'Shea

Publication

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of glgC gene)

Use

Cloning vector

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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glgA-

Plasmid

#101841

Purpose

deletes glgA locus

Depositor

Erin O'Shea

Publication

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of glgA gene)

Use

Cloning vector

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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gnd-

Plasmid

#101840

Purpose

deletes gnd locus

Depositor

Erin O'Shea

Publication

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of gnd gene)

Use

Cloning vector

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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POT2-CYC1p-ycrtE-ADH1t

Plasmid

#65354

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtE-ADH1t

Use

Unspecified

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT4-CYC1p-ycrtI-ADH1t

Plasmid

#65357

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtI-ADH1t

Use

Unspecified

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT4-TEF2p-ycrtI-ADH1t

Plasmid

#65356

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtI-ADH1t

Use

Unspecified

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-P-TEF2

Plasmid

#65337

Purpose

construction of TEF2 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2 promoter

Use

Synthetic Biology

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Rab-Rep

Plasmid

#45967

Depositor

Ralf Kuehn

Insert

beta-Galactosidase

Expression

Mammalian

Mutation

duplicated aa 1-135

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pSA25

Plasmid

#35116

Depositor

John McCusker

Publication

Ito-Harashima et al Yeast. 2004 Jan 15;21(1):53-61.

Insert

lys5MX4

Use

Yeast replicating

Available since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBN62

Plasmid

#35105

Depositor

John McCusker

Publication

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fcy1PR

Use

Yeast replicating

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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Ntom-Venus-ssrA

Plasmid

#223688

Purpose

PhoBIT1 component; Venus-tagged ssrA tag fused with Ntom for mitochondrial tethering

Depositor

Yubin Zhou

Publication

Lee et al Nat Commun. 2025 Jul 28;16(1):6940. doi:

Insert

ssrA peptide

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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