We narrowed to 14,090 results for: cas9

1. pAP607-2

   Plasmid

   #99483

   Purpose

   Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc
   

   Expression

   Bacterial

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWT019b

   Plasmid

   #107894

   Purpose

   W1.2 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C) and is part of CAMERA 1.2

   Depositor

   David Liu

   Article

   Tang et al Science. 2018 Feb 15. pii: science.aap89

   Insert

   PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C)
   

   Expression

   Bacterial

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. BCJJ223

   Plasmid

   #117509

   Purpose

   AtMGE1 promoter Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:GGAG:pMGE1:AATG:BsaI
   

   Expression

   Plant

   Promoter

   AtMGE1

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICSL12021

   Plasmid

   #117511

   Purpose

   AtICU2 promoter Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:GGAG:pICU2:AATG:BsaI
   

   Expression

   Plant

   Promoter

   AtICU2

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAP684-3

   Plasmid

   #99489

   Purpose

   TEV::eGFP::ER::Ollas::linker::3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   TEV::eGFP::RE::Ollas::linker::3XFlag
   

   Expression

   Bacterial

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBHM2674

   Plasmid

   #241727

   Purpose

   Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BSR marker for selection on blasticidin S

   Depositor

   Hiten Madhani

   Article

   Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Sept. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBHM2672

   Plasmid

   #241719

   Purpose

   Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BLE marker for selection on phleomycin

   Depositor

   Hiten Madhani

   Article

   Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Sept. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAT22

   Plasmid

   #204311

   Purpose

   For generating targeting inversions. N-terminal split GFP, expressed in eyes.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-GFPn
   

   Use

   CRISPR

   Promoter

   3XP3

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. psBIG1a ActBT2ATetP2AP - mAGH

   Plasmid

   #229973

   Purpose

   Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBT2ATetP2AP - mAGH
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. psBIG1a ActBT2ATetP2AP - H

    Plasmid

    #229972

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2ATetP2AP - H
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. psBIG1a ActBT2AmcherryP2AP -TagBFPH

    Plasmid

    #229971

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2AmcherryP2AP -TagBFPH
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222857

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(CTG)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgCTG
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222859

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(AGC)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgAGC
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222858

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(CAG)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgCAG
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222860

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(GCA)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgGCA
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMLS713

    Plasmid

    #188885

    Purpose

    Multisite Gateway [4-3] Destination vector; miniMos transposon with lox2272-flanked Cbr-unc-119 marker

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Type

    Empty backbone

    Use

    Multisite gateway [4-3] destination vector

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOC5 cloning template vector

    Plasmid

    #183424

    Purpose

    FlpOFF knock-in vector

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOC3 cloning template vector

    Plasmid

    #183422

    Purpose

    CreON FlpOFF knock-in vector

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKEW395_AcrIIA14_truncated

    Plasmid

    #138284

    Purpose

    Expression of truncated AcrIIA14 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    truncated AcrIIA14
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKEW398_AcrIIA15_truncated

    Plasmid

    #138286

    Purpose

    Expression of truncated AcrIIA15 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA15
    

    Use

    CRISPR

    Tags

    SFGFP

    Expression

    Bacterial

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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