We narrowed to 11,769 results for: 110

1. pFRT-TODestFLAGHA_RBPMS

   Plasmid

   #59388

   Purpose

   Destination vector with RBPMS for stable cell line generation

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Promoter

   CMV, 2x Tet

   Available Since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMMP-NGFR:LMP1-IRES-mRFP

   Plasmid

   #36974

   Depositor

   Bill Sugden

   Article

   Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

   Insert

   NGFR:LMP1 (NGFR, LMP-1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   NGFR:LMP1 chimeric receptor consists of aa 1–276 …

   Promoter

   CMV

   Available Since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRAF_S29A_pcDNA3.1

   Plasmid

   #86506

   Purpose

   Expresses CRAF_S29A in mammalian cells [Edit]

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   CRAF (RAF1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S29A

   Promoter

   CMV

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIBN-TetR-tagRFP-T

   Plasmid

   #103809

   Purpose

   'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-TetR-tagRFP-T (CIB1, tetR Mustard Weed, E. coli)
   

   Expression

   Mammalian

   Mutation

   TetR: A4G (M2V)

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. mIFP-Actin-C-18

   Plasmid

   #56211

   Purpose

   Localization: Actin, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   NM_001101.3 (ACTB Human)
   

   Tags

   mIFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pET28a-FgßF3-ddFLN4-HIS-ybbr

   Plasmid

   #101743

   Purpose

   mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   FgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
   

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4, added 9F in FgßF3

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFB-U6-Chrna3-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128338

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrna3 (Chrna3 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

   Plasmid

   #166796

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   KK93EE

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

   Plasmid

   #166800

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   E597R

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.1Xpress-BcrGAP R1090A

    Plasmid

    #38176

    Depositor

    Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    BCR GAP domain encoding residues 1004-1271 with R1090A point mutation (BCR Human)
    

    Tags

    Xpress

    Expression

    Mammalian

    Mutation

    aa 1004-1271 with R1090A point mutation

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pFB-U6-Chrna2-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128337

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna2 (Chrna2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6_sgRNA_CAG_hSt1Cas9_CNRZ1066

    Plasmid

    #136651

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9:CNRZ_1066 Chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and hU6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PIK3CD gRNA (BRDN0001146527)

    Plasmid

    #76164

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3CD

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. integrin beta6 777T pBabe puro

    Plasmid

    #13583

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

15. GFP-RanV19 E1RA2

    Plasmid

    #118317

    Purpose

    Expression of human Ran

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Inserts

    RanV19 (RAN Human)
    EPac1RA2 (RAPGEF3, RAPGEF4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    in-frame insertion of RanV19 into GFPEpac1RA2 and…

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_KLF12_P2A_Hygro_Barcode

    Plasmid

    #120498

    Purpose

    Barcoded lentiviral vector to express KLF12 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF12 (KLF12 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG mFoxg1-GFP T271E

    Plasmid

    #73057

    Purpose

    C-terminal GFP fusion of Mouse Foxg1 T271E

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    Foxg1 (Foxg1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T271E

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG mFoxg1-GFP T271A

    Plasmid

    #73047

    Purpose

    C-terminal fusion of gfp Mouse Foxg1 T271A

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    Foxg1 (Foxg1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T271A

    Promoter

    CAG

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-crRNA(TBK1)

    Plasmid

    #109322

    Purpose

    AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    mCherry-KASH (TBK1 Human)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Rb-379-928Δ703-737-pGex2T

    Plasmid

    #21933

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

    Insert

    Rb (RB1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-928D703-737 o…

    Available Since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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