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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,523 results for: bli

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Showing: 5041 - 5060 of 15523 results

  1. pTRIPZ (M)-HT-Cbx7-ATLm

    Plasmid
    #82522
    Purpose
    Lentivirus vector. Expresses mutant Halotag-Cbx7-ATLm fusion proteins in mammalian cells. Cbx7 ATL mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    mutation of RKR70-72, K75, and KR77-78 to AGA70-7…
    Promoter
    CMV
    Available Since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82522-plasmid-map-sequence-id-156808

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  2. pJK371

    Plasmid
    #73596
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 3)
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Point mutants in FAD2-3 ORF relative to deposited…
    Promoter
    GAL1 and GAL10
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73596-plasmid-map-sequence-id-139406

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  3. pGEX2TK-YAP-WW1

    Plasmid
    #104305
    Purpose
    bacterial expression of WW domain 1 of YAP; binds PPxY where Y is not phosphorylated
    Depositor
    Marius Sudol
    Insert
    YAP WW1 domain (YAP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    WW domain 1
    Promoter
    tac
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104305-plasmid-map-sequence-id-201233

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  5. HECW2

    Plasmid
    #32875
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HECW2 (HECW2 Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. 4IA9

    Plasmid
    #51320
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5 (WDR5 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. Hs.KRAS4b C185S

    Plasmid
    #83139
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), C185S mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    C185S
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83139-plasmid-map-sequence-id-158102

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  9. 4PZI

    Plasmid
    #61955
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MLL4:JMC054-D08:C226821 (KMT2B Human)
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. PIWIL2

    Plasmid
    #32042
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PIWIL2 (PIWIL2 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    PAZ domain only (amino acids 386-533)
    Available Since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. EPB41

    Plasmid
    #32040
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EPB41 (EPB41 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    FERM domain only (amino acids 1-279)
    Available Since
    Jan. 13, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. HSD17B8

    Plasmid
    #38853
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B8 (Hsd17b8 Human)
    Tags
    Tev His Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. GHRHR

    Plasmid
    #39173
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GHRHR (GHRHR Human)
    Use
    Baculovirus expression
    Tags
    Signal-His6-TEV
    Mutation
    N20->Q mutation of putative glycosylsation sit…
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. Hs.KRAS4b Q61K

    Plasmid
    #83164
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    Q61K
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83164-plasmid-map-sequence-id-158127

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  16. Gli3ZF in AINGpCAGGS

    Plasmid
    #121972
    Purpose
    Gli3 zinc fingers
    Depositor
    James Briscoe
    Insert
    Gli3ZF (GLI3 Human)
    Use
    Chick electroporation
    Tags
    5xmyc
    Expression
    Mammalian
    Mutation
    N terminus and C terminus deletion only nt 1411-1…
    Available Since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    121972-plasmid-map-sequence-id-241924

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  17. pAdTrack-mLIP

    Plasmid
    #49197
    Purpose
    adenoviral shuttle vector with mouse C/EBPbeta LIP fragment
    Depositor
    Jed Friedman
    Insert
    C/EBPbeta (LIP fragment) (Cebpb Mouse)
    Use
    Adenoviral
    Mutation
    only the LIP fragment
    Promoter
    CMV
    Available Since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. Myc-gamma2s-EGFP

    Plasmid
    #118040
    Purpose
    GABAA receptor expression (gamma2 short subunit) double tagged, near N terminus and at C-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG2 (Gabrg2 Rat)
    Tags
    EGFP and cMyc epitope EQKLISEEDL inserted between…
    Expression
    Mammalian
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    118040-plasmid-map-sequence-id-228639

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  19. pcDNA5/FRT/TO/Flag-tGFP-Cryz

    Plasmid
    #110378
    Purpose
    Mammalian expression of turboGFP-CRYZ with FLAG tag, Flp-In T-Rex system
    Depositor
    Sandra Martha Gomes Dias
    Insert
    crystallin zeta (CRYZ Human)
    Tags
    FLAG and tGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110378-plasmid-map-sequence-id-213403

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  20. pDNR-hSox2

    Plasmid
    #49389
    Purpose
    donor vector for cloning human Sox2 with 6xHN tag
    Depositor
    Randall McKinnon
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Donor vector
    Tags
    6xHN (6 repeating His-Asn subunits)
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    none
    Available Since
    Nov. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 5041 - 5060 of 15523 results